Duplex StabilizationEpigenetics/DNA Methylation
Serinol COT-Serinol Dabcyl Biotin Fluorescein Rhodamine Cyanine Elitechgroup Dyes and Quencher Black Hole Quencher Blackberry Rhodamine (TAMRA) Acridine DNP Cholesterol Tocopherol Stearyl Psoralen EDTA Ferrocene Methylene Blue Metal Chelates Polyaromic Hydrocarbons Puromycin Quenched Autoligation (QUAL) Probes Photo Regulation
RNA Supports For 3'-Modification TOM-Protected RNA Amidites RNA Supports For TOM RNA Synthesis TBDMS-Protected RNA Amidites HT RNA Amidites UltraMild TBDMS RNA Amidites TBDMS RNA Supports UltraMild Solvents/Reagents Minor RNA Amidites (TOM) RNA Sequence Modifier (TOM-Protected) Minor RNA Amidites (TBDMS-Protected) Minor RNA Triphosphates 2'-OMe-RNA Amidites 2'-OMe-Supports HT 2'-OMe-RNA Amidites Ultramild 2'-OME-RNA Minor 2'-OMe-RNA Amidites 2'-OMe-Thiophosphoramidites 2'-F-RNA Monomers 2'-F-Arabinonucleic Acid (2'-F-ANA)
NAIM (Catalog as PDF)
A AnalogsNucleotide Analog Interference Mapping (NAIM) is a chemogenetic approach that makes it possible to simultaneously, yet individually, probe the contribution of a particular functional group at almost every RNA nucleotide position in a single experiment1,2. The method utilizes a series of 5'-O-(1-thio)nucleoside analog triphosphates in a modification interference procedure that is as simple as RNA sequencing. In a NAIM experiment the smallest mutable unit is not the base pair, but rather the individual functional groups that comprise the nucleotides. Because the modification or deletion of a particular functional group within an RNA can severely affect its activity, this approach makes it possible to efficiently determine the chemical basis of RNA structure and function.
NAIM utilizes nucleotide analogs, each of which includes an incremental chemical alteration in the base or ribose sugar. The most completely developed set of analogs are those of adenosine, for which eight different analogs have been utilized in NAIM.3 Five analogs modify the nucleotide base and three modify the ribose sugar. The base analogs include purine riboside (PuraS), N6-methyladenosine (m6AaS), tubercidin (7dAaS), diaminopurine riboside (DAPaS), and 2-aminopurine riboside (2APaS). The ribose sugar analogs all modify the 2'-OH group and include 2'-deoxyadenosine (dAaS), 2'-deoxy-2'-fluoroadenosine (FAaS), and 2'-O-methyladenosine (OMeAaS). All of the analogs can be randomly incorporated into an RNA transcript at an ideal 5% level of efficiency using either the wild-type T7 RNA polymerase or a Y639F RNA polymerase point mutant4. Each of these analogs provides specific information about the chemical basis of RNA activity at almost every incorporated position in the transcript.
α-Thiotriphosphates are sodium salts in TE buffer, pH7, 10X concentrates. The concentrations shown are optimal for incorporation during polymerase reactions.
Products for Nucleotide Analog Interference Mapping (NAIM) are supplied under license.