Mermade Instruments (Catalog as PDF)

Sterling CE Phosphoramidites

MerMade synthesizers belong to a family of synthesizers, including the column-based MerMade 4, MerMade 6 and 12 instruments and the parallel array synthesizers, MerMade 192 and MerMade 192E, manufactured by BioAutomation Corporation in Plano, TX. Their web site can be found at:http://www.BioAutomation.com. Phosphoramidite monomers are packaged in 30mL and 240mL amber bottles for dissolving at a concentration of 1g/20mL and are connected directly to the instrument. Some instruments may also be configured to accept Applied Biosystems serum vials, as shown on page 6.
Item Catalog No. Pack Price ($)
10-1000-02M 0.25g 12.50
10-1000-05M 0.5g 25.00
10-1000-10M 1.0g 50.00
10-1000-5S 5.0g 250.00
10-1000-1S 10.0g 500.00
10-1010-02M 0.25g 12.50
10-1010-05M 0.5g 25.00
10-1010-10M 1.0g 50.00
10-1010-5S 5.0g 250.00
10-1010-1S 10.0g 500.00
10-1015-02M 0.25g 12.50
10-1015-05M 0.5g 25.00
10-1015-10M 1.0g 50.00
10-1015-5S 5.0g 250.00
10-1015-1S 10.0g 500.00
10-1020-02M 0.25g 12.50
10-1020-05M 0.5g 25.00
10-1020-10M 1.0g 50.00
10-1020-5S 5.0g 250.00
10-1020-1S 10.0g 500.00
10-1029-02M 0.25g 12.50
10-1029-05M 0.5g 25.00
10-1029-10M 1.0g 50.00
10-1029-5S 5.0g 250.00
10-1029-1S 10.0g 500.00
10-1030-02M 0.25g 12.50
10-1030-05M 0.5g 25.00
10-1030-10M 1.0g 50.00
10-1030-5S 5.0g 250.00
10-1030-1S 10.0g 500.00
dA
dA
dC
dC
Ac-dC
Ac-dC
dG
dG
dmf-dG
dmf-dG
dT
dT

QUALITY ASSURANCE

Every batch of these CE Phosphoramidites is tested as follows

  1. HPLC
    a) Identity is confirmed by comparison with a reference sample.
    b) Purity is determined by HPLC to be ≥98.0%.
  2. TLC
    Purity is verified by TLC.
  3. 31P NMR
    Purity is determined by 31P NMR to be ≥98%.
  4. Coupling Test
    Coupling efficiency is determined to be ≥99%.
  5. Solution Test
    A 0.1M solution is determined to be clear and free of particulate contamination.
  6. Loss on Drying
    Volatile contaminants are determined to be ≤2%.

Sterling Solvents/Reagents

All solvents and reagents are prepared to our exacting specifications to ensure the highest synthesis efficiency and are passed through a 0.2 micron filter during packaging to eliminate particulate contamination. Parallel synthesizers typically use 5-ethylthio-1H-tetrazole (ETT) as activator to minimize the chance of crystallization. ETT is used at a concentration of 0.25M in acetonitrile, which is far below the level at which crystallization may occur.
Item Catalog No. Pack Price ($)
Activator
30-3140-57 450mL 200.00
30-3140-61 960mL 365.00
30-3140-62 2000mL 760.00
Diluent
40-4050-50 100mL 16.00
Cap Mix A
40-4010-57 450mL 72.00
40-4010-61 960mL 154.00
40-4010-62 2000mL 325.00
Cap Mix B
40-4220-57 450mL 96.00
40-4220-61 960mL 204.00
40-4220-62 2000mL 425.00
Ozidizing Solution
40-4330-57 450mL 72.00
40-4330-61 960mL 154.00
40-4330-62 2000mL 325.00
Deblocking Mix
40-4040-57 450mL 36.00
40-4040-61 960mL 75.00
40-4040-62 2000mL 144.00
40-4140-57 450mL 36.00
40-4140-61 960mL 75.00
40-4140-62 2000mL 144.00

ABBREVIATIONS

Ac2O = Acetic Anhydride
CE = Cyanoethyl
CPG = Controlled Pore Glass
DCA = Dichloacetic Acid
DCM = Dichloromethane
DMAP = Dimethylaminopyridine
dmf = dimethylformamidine
DMT = 4,4’-Dimethoxytrityl
I2 = Iodine
iPr = Isopropyl
MeIm = 1-Methylimidazole
µm = micromole(s)
nm = nanomole(s)
Pac = Phenoxyacetyl
PhOAc = Phenoxyacetyl
TCA = Trichloroacetic Acid
THF = Tetrahydrofuran

See Also

Sterling Supports

Columns containing 1000Å CPG are available in packs of 200 to fit MerMade plates. Regular 500Å or 1000Å supports, listed on page 8, may also be used to fill the wells of regular 96 well plates. However, this requires each plate to be prepared with each nucleoside accurately in all wells. A universal support clearly removes the need for four specific supports and makes preparing plates straightforward. Glen UnySupport™ 40 nmole frits, as described onpage 22, can also be used.

Columns containing 1000Å CPG are available in packs of 200 to fit MerMade plates. Regular 500Å or 1000Å supports, listed on page 8, may also be used to fill the wells of regular 96 well plates. However, this requires each plate to be prepared with each nucleoside accurately in all wells. A universal support clearly removes the need for four specific supports and makes preparing plates straightforward. Glen UnySupport™ 40 nmole frits, as described onpage 22, can also be used.

Catalog No. Catalog No. Catalog No. Catalog No. Catalog No. Catalog No. Pack Price($)
dA dC dG dT Ac-dC dmf-dG    
Mermade 1000Å Columns
20-2001-65 20-2021-65 20-2031-65 20-2015-65 20-2029-65 200x50nm 750.00
20-2001-62 20-2021-62 20-2031-62 20-2015-62 20-2029-62 200x200nm 750.00
20-2001-61 20-2021-61 20-2031-61 20-2015-61 20-2029-61 48x1.0µm 300.00
dA-CPG 1000
dA-CPG 1000
dG-CPG 1000
dG-CPG 1000
dT-CPG 1000
dT-CPG 1000
Ac-dC-CPG 1000
Ac-dC-CPG 1000
dmf-dG-CPG
dmf-dG-CPG

See Also

Supports (Part 2)

Item Catalog No. Pack Price ($)
Glen UnySupport 1000
20-5141-91 Pack of 96 375.00
  200 nmole columns 20-5141-92 Pack of 96 250.00
  40 nmole columns 20-5141-95 Pack of 96 250.00
Empty MerMade Columns
20-0050-05 Pack of 48 200.00
20-0050-02 Pack of 48 200.00
Glen UnySupport™ 1000
Glen UnySupport™ 1000

Intellectual Property

Glen UnySupport 1000 is covered by US Patent 7,202,264 owned by Ionis Pharmaceuticals, Inc..


Contact to place an order.
INTERNATIONAL USERS: Contact your distributor or order from USA
Contact to access more than 50 years of oligo expertise for help in designing oligos for your unique application.
Glen Research Corporation, Copyright © 2019