Expedite Instruments (Catalog as PDF)

Sterling CE Phosphoramidites

Glen Research CE (β-cyanoethyl) Phosphoramidites are produced and packaged to ensure the highest performance on DNA synthesizers. Every Glen Research product is accompanied by a Certificate of Analysis and HPLC trace, showing the results of our QC testing. Every Glen Research monomer vial is specially cleaned to eliminate particulate contamination.
Item Catalog No. Pack Price ($)
10-1000-C2 0.25g 12.50
10-1000-C5 0.5g 25.00
10-1000-1C 1.0g 50.00
10-1000-2C 2.0g 100.00
10-1010-C2 0.25g 12.50
10-1010-C5 0.5g 25.00
10-1010-1C 1.0g 50.00
10-1010-2C 2.0g 100.00
10-1015-C2 0.25g 12.50
10-1015-C5 0.5g 25.00
10-1015-1C 1.0g 50.00
10-1015-2C 2.0g 100.00
10-1020-C2 0.25g 12.50
10-1020-C5 0.5g 25.00
10-1020-1C 1.0g 50.00
10-1020-2C 2.0g 100.00
10-1029-C2 0.25g 12.50
10-1029-C5 0.5g 25.00
10-1029-1C 1.0g 50.00
10-1029-2C 2.0g 100.00
10-1030-C2 0.25g 12.50
10-1030-C5 0.5g 25.00
10-1030-1C 1.0g 50.00
10-1030-2C 2.0g 100.00
dA
dA
dC
dC
Ac-dC
Ac-dC
dG
dG
dmf-dG
dmf-dG
dT
dT

QUALITY ASSURANCE

Every batch of these CE Phosphoramidites is tested as follows

  1. HPLC
    a) Identity is confirmed by comparison with a reference sample.
    b) Purity is determined by HPLC to be ≥98.0%.
  2. TLC
    Purity is verified by TLC.
  3. 31P NMR
    Purity is determined by 31P NMR to be ≥98%.
  4. Coupling Test
    Coupling efficiency is determined to be ≥99%.
  5. Solution Test
    A 0.1M solution is determined to be clear and free of particulate contamination.
  6. Loss on Drying
    Volatile contaminants are determined to be ≤2%.

Sterling Solvents/Reagents

All solvents and reagents are prepared to our exacting specifications to ensure the highest synthesis efficiency and are passed through a 0.2 micron filter during packaging to eliminate particulate contamination. Glen Research uses freshly sublimed 1H-tetrazole for premium performance on Expedite synthesizers. Crystalline tetrazole solutions have been discontinued.
Item Catalog No. Pack Price ($)
Activator
30-3102-66 1 60mL 50.00
30-3102-52 2 200mL 100.00
30-3100-57 2 450mL 200.00
Diluent
40-4050-45 60mL 12.00
40-4050-50 100mL 16.00
Anhydrous Wash
40-4050-53 1 300mL 40.00
40-4050-57 2 450mL 50.00
Cap Mix A
40-4012-66 1 60mL 15.00
Cap Mix A
40-4012-66 1 60mL 15.00
40-4012-52 2 200mL 30.00
40-4012-57 2 450mL 72.00
Cap Mix B
40-4122-66 1 60mL 20.00
40-4122-52 2 200mL 40.00
40-4122-57 2 450mL 96.00
Oxidizing Solution
40-4132-66 1 60mL 20.00
40-4132-52 2 200mL 40.00
40-4132-57 2 450mL 96.00
Deblocking Mix
40-4140-68 1 180mL 18.00
40-4140-71 2 1L 80.00

EXPEDITE INSTRUMENTS


  1. For use on Expedite 8905
    instruments.
  2. For use on Expedite 8909
    instruments.

ABBREVIATIONS

Ac2O = Acetic Anhydride
CE = Cyanoethyl
CPG = Controlled Pore Glass
DCA = Dichloacetic Acid
DCM = Dichloromethane
DMAP = Dimethylaminopyridine
dmf = dimethylformamidine
DMT = 4,4’-Dimethoxytrityl
I2 = Iodine
iPr = Isopropyl
MeIm = 1-Methylimidazole
µm = micromole(s)
nm = nanomole(s)
Pac = Phenoxyacetyl
PhOAc = Phenoxyacetyl
TCA = Trichloroacetic Acid
THF = Tetrahydrofuran

See Also

Sterling Supports

All Glen Research supports use the standard long chain alkylamino (lcaa) linker but differ in the glass pore size, 500Å, 1000Å or 2000Å. The 500Å support is appropriate for shorter sequences, while the 1000Å supports perform better in the synthesis oflonger (>30-mer) DNA sequences. The 2000Å support is best for very long (>150-mer) oligonucleotides. We have instituted an additional QC test for supports to show the length of oligo that can be prepared before a drop-off in coupling due to steric effects begins to occur. The drop-off point is recorded in the Certificate of Analysis. All Glen Research supports are fully end-capped to ensure that the CPG surface is totally inert, thereby avoiding the introduction of impurity sequences containing deletions at the 3'-terminus.
Catalog No. Catalog No. Catalog No. Catalog No. Catalog No. Catalog No. Catalog No. Pack Price($)
dA dC dG dT dA,dC,dG,dT
1 of
each base
Ac-dC dmf-dG    
500Å Columns
20-2200-42 20-2210-42 20-2220-42 20-2230-42 20-2240-42 20-2213-42 4x0.2µm 40.00
20-2200-41 20-2210-41 20-2220-41 20-2230-41 20-2240-41 20-2213-41 4x1.0µm 60.00
20-2200-14 20-2210-14 20-2220-14 20-2230-14 20-2213-14 1x15µm 150.00
1000Å Columns
20-2201-45 20-2211-45 20-2221-45 20-2231-45 20-2241-45 20-2215-45 20-2229-45 4x40nm 40.00
20-2201-42 20-2211-42 20-2221-42 20-2231-42 20-2241-42 20-2215-42 20-2229-42 4x0.2µm 40.00
20-2201-41 20-2211-41 20-2221-41 20-2231-41 20-2241-41 20-2215-41 20-2229-41 4x1.0µm 60.00
20-2201-14 20-2211-14 20-2221-14 20-2231-14 20-2215-14 20-2229-14 1x15µm 150.00
2000Å Columns
20-2202-42 20-2212-42 20-2222-42 20-2232-42 20-2242-42 4x0.2µm 40.00
500Å Bulk CPG
20-2000-01 20-2010-01 20-2020-01 20-2030-01 20-2013-01 0.1g 9.00
20-2000-02 20-2010-02 20-2020-02 20-2030-02 20-2013-02 0.25g 20.00
20-2000-10 20-2010-10 20-2020-10 20-2030-10 20-2013-10 1.0g 75.00
1000Å Bulk CPG
20-2001-01 20-2011-01 20-2021-01 20-2031-01 20-2015-01 20-2029-01 0.1g 9.00
20-2001-02 20-2011-02 20-2021-02 20-2031-02 20-2015-02 20-2029-02 0.25g 20.00
20-2001-10 20-2011-10 20-2021-10 20-2031-10 20-2015-10 20-2029-10 1.0g 75.00
2000Å Bulk CPG
20-2002-01 20-2012-01 20-2022-01 20-2032-01 0.1g 15.00
20-2002-02 20-2012-02 20-2022-02 20-2032-02 0.25g 30.00
20-2002-10 20-2012-10 20-2022-10 20-2032-10 1.0g 105.00
dA-CPG 500
dA-CPG 500
dC-CPG 500
dC-CPG 500
dG-CPG 500
dG-CPG 500
dT-CPG 500
dT-CPG 500

Ac-dC-CPG 500
Ac-dC-CPG 500
dA-CPG 1000
dA-CPG 1000
dC-CPG 1000
dC-CPG 1000
dG-CPG 1000
dG-CPG 1000
dT-CPG 1000
dT-CPG 1000
Ac-dC-CPG 1000
Ac-dC-CPG 1000
dmf-dG-CPG
dmf-dG-CPG
dA-CPG 2000
dA-CPG 2000
dC-CPG 2000
dC-CPG 2000
dG-CPG 2000
dG-CPG 2000

BULK CPG LOADING

500Å supports 35-50µmoles/g
1000Å supports 25-40µmoles/g

See Also

Empty columns and frits

Item Catalog No. Pack Price ($)
20-0021-02 Pack of 10 48.00
20-0021-01 Pack of 10 48.00
20-0021-0F Pack of 20 20.00
20-0040-00 Pack of 10 300.00
20-0040-0F Pack of 20 30.00

Contact to place an order.
INTERNATIONAL USERS: Contact your distributor or order from USA
Contact to access more than 50 years of oligo expertise for help in designing oligos for your unique application.
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