Duplex StabilizationEpigenetics/DNA Methylation
Serinol COT-Serinol Dabcyl Biotin Fluorescein Rhodamine Cyanine Elitechgroup Dyes and Quencher Black Hole Quencher Blackberry Rhodamine (TAMRA) Acridine DNP Cholesterol Tocopherol Stearyl Psoralen EDTA Ferrocene Methylene Blue Metal Chelates Polyaromic Hydrocarbons Puromycin Quenched Autoligation (QUAL) Probes Photo Regulation
RNA Supports For 3'-Modification TOM-Protected RNA Amidites RNA Supports For TOM RNA Synthesis TBDMS-Protected RNA Amidites HT RNA Amidites UltraMild TBDMS RNA Amidites TBDMS RNA Supports UltraMild Solvents/Reagents Minor RNA Amidites (TOM) RNA Sequence Modifier (TOM-Protected) Minor RNA Amidites (TBDMS-Protected) Minor RNA Triphosphates 2'-OMe-RNA Amidites 2'-OMe-Supports HT 2'-OMe-RNA Amidites Ultramild 2'-OME-RNA Minor 2'-OMe-RNA Amidites 2'-OMe-Thiophosphoramidites 2'-F-RNA Monomers 2'-F-Arabinonucleic Acid (2'-F-ANA)
Epigenetics (Catalog as PDF)
DNA MethylationOne of the fastest growing fields in biology and cancer research is epigenetics. While the underlying genetic code defines which proteins and gene products are synthesized, it is epigenetic control that defines when and where they are expressed. This dynamic control of gene expression is essential for X chromosome inactivation, embryogenesis, cellular differentiation and appears integral to memory formation and synaptic plasticity.
In 2009, two reports1,2 described the discovery of 5-hydroxymethyl-2'-deoxyCytidine (hmdC), a novel dC modification in Purkinje neurons and embryonic stem cells. Later, a third report found this modification to be strongly enriched in brain tissues associated with higher cognitive functions.3 This dC modification is generated by the action of α-ketoglutarate dependent ten eleven translocation (TET) enzymes, which oxidizes 5-Me-dC to hmdC. This finding stimulated discussion about active demethylation pathways that could occur, e.g., via base excision repair (BER), with the help of specialized DNA glycosylases. Alternatively, one could envision a process in which the hydroxymethyl group of hmdC is further oxidized to 5-formyl-dC (fdC) or 5-carboxy-dC (cadC) followed by elimination of either formic acid or carbon dioxide4,5. Glen Research has supported this research since its inception by providing the building blocks for the synthesis of oligonucleotides containing all the new dC derivatives - hmdC, fdC and cadC. The first generation hmdC phosphoramidite was fairly very well accepted but requires fairly harsh deprotection conditions. Therefore, a second generation building block (5-Hydroxymethyl-dC II) developed by Carell and co-workers that is compatible with UltraMild deprotection was introduced.6 5-Formy-dC III has been designed to meet all of the requirements to prepare an oligo containing all of the methylated variants.7
5-Formyl dC III CE
OTHER INSTRUMENT TYPES
All minor bases, RNA products and modifiers are packaged in septumcapped vials suitable for ABI and other instruments. If you would like another type of vial/column add the following to the end of the catalog number.