Dr. Oligo (Catalog as PDF)

Sterling CE Phosphoramidites

Dr. Oligo synthesizers belong to a family of synthesizers, including the parallel array synthesizers, Dr. Oligo 96, Dr. Oligo 192, Dr. Oligo 384 and Dr. Oligo 768, manufactured by Biolytic® Lab Performance, Inc. in Fremont, CA. Their web site can be found at: http://www.biolytic.com. Phosphoramidite monomers are packaged in 30mL and 240mL amber bottles for dissolving at a concentration of 1g/20mL and are connected directly to the instrument. Some instruments may also be configured to accept Applied Biosystems serum vials, as shown on page 6.
Item Catalog No. Pack Price ($)
10-1000-02M 0.25g 12.50
10-1000-05M 0.5g 25.00
10-1000-10M 1.0g 50.00
10-1000-5S 5.0g 250.00
10-1000-1S 10.0g 500.00
10-1010-02M 0.25g 12.50
10-1010-05M 0.5g 25.00
10-1010-10M 1.0g 50.00
10-1010-5S 5.0g 250.00
10-1010-1S 10.0g 500.00
10-1015-02M 0.25g 12.50
10-1015-05M 0.5g 25.00
10-1015-10M 1.0g 50.00
10-1015-5S 5.0g 250.00
10-1015-1S 10.0g 500.00
10-1020-02M 0.25g 12.50
10-1020-05M 0.5g 25.00
10-1020-10M 1.0g 50.00
10-1020-5S 5.0g 250.00
10-1020-1S 10.0g 500.00
10-1029-02M 0.25g 12.50
10-1029-05M 0.5g 25.00
10-1029-10M 1.0g 50.00
10-1029-5S 5.0g 250.00
10-1029-1S 10.0g 500.00
10-1030-02M 0.25g 12.50
10-1030-05M 0.5g 25.00
10-1030-10M 1.0g 50.00
10-1030-5S 5.0g 250.00
10-1030-1S 10.0g 500.00
dA
dA
dC
dC
Ac-dC
Ac-dC
dG
dG
dmf-dG
dmf-dG
dT
dT

QUALITY ASSURANCE

Every batch of these CE Phosphoramidites is tested as follows

  1. HPLC
    a) Identity is confirmed by comparison with a reference sample.
    b) Purity is determined by HPLC to be ≥98.0%.
  2. TLC
    Purity is verified by TLC.
  3. 31P NMR
    Purity is determined by 31P NMR to be ≥98%.
  4. Coupling Test
    Coupling efficiency is determined to be ≥99%.
  5. Solution Test
    A 0.1M solution is determined to be clear and free of particulate contamination.
  6. Loss on Drying
    Volatile contaminants are determined to be ≤2%.

Sterling Solvents/Reagents

All solvents and reagents are prepared to our exacting specifications to ensure the highest synthesis efficiency and are passed through a 0.2 micron filter during packaging to eliminate particulate contamination. Parallel synthesizers typically use 5-ethylthio-1H-tetrazole (ETT) as activator to minimize the chance of crystallization. ETT is used at a concentration of 0.25M in acetonitrile, which is far below the level at which crystallization may occur.
Item Catalog No. Pack Price ($)
Activator
30-3140-57 450mL 200.00
30-3140-62 2000mL 760.00
Diluent
40-4050-50 100mL 16.00
Cap Mix A
40-4010-57 450mL 72.00
40-4010-62 2000mL 325.00
Cap Mix B
40-4220-57 450mL 96.00
40-4220-62 2000mL 425.00
Oxidizing Solution
40-4330-57 450mL 72.00
40-4330-62 2000mL 325.00
Deblocking Mix
40-4040-57 450mL 36.00
40-4040-62 2000mL 144.00
40-4140-57 450mL 36.00
40-4140-62 2000mL 144.00

ABBREVIATIONS

Ac2O = Acetic Anhydride
CE = Cyanoethyl
CPG = Controlled Pore Glass
DCA = Dichloacetic Acid
DCM = Dichloromethane
DMAP = Dimethylaminopyridine
dmf = dimethylformamidine
DMT = 4,4’-Dimethoxytrityl
I2 = Iodine
iPr = Isopropyl
MeIm = 1-Methylimidazole
µm = micromole(s)
nm = nanomole(s)
Pac = Phenoxyacetyl
PhOAc = Phenoxyacetyl
TCA = Trichloroacetic Acid
THF = Tetrahydrofuran

See Also

Sterling Supports

Dr. Oligo instruments are designed for flexibity in the use of supports and columns. They can use fritted plates with loose CPG (page 8) and AB 3900 style polystyrene and CPG columns. Glen UnySupport™ 40 nmole frits can also be used.

Dr. Oligo instruments are designed for flexibity in the use of supports and columns. They can use fritted plates with loose CPG (page 8) and AB 3900 style polystyrene and CPG columns. Glen UnySupport™ 40 nmole frits can also be used.

Catalog No. Catalog No. Catalog No. Catalog No. Catalog No. Catalog No. Pack Price($)
dA dC dG dT Ac-dC dmf-dG    
AB 3900 Polystyrene Columns
26-2600-65 26-2610-65 26-2630-65 26-2629-65 200x40nm 825.00
26-2600-62 26-2610-62 26-2630-62 26-2629-62 200x200nm 825.00
AB 3900 1000Å CPG Columns
20-2101-65 20-2131-65 20-2115-65 20-2129-65 200x40nm 600.00
20-2101-62 20-2131-62 20-2115-62 20-2129-62 200x200nm 650.00
20-2101-61 20-2131-61 20-2115-61 20-2129-61 200x1.0µm 875.00
dA-CPG 1000
dA-CPG 1000
dT-CPG 1000
dT-CPG 1000
Ac-dC-CPG 1000
Ac-dC-CPG 1000
dmf-dG-CPG
dmf-dG-CPG
dA PS
dA PS
dC PS
dC PS
dT PS
dT PS
dmf-dG PS
dmf-dG PS

See Also

OLIGONUCLEOTIDE PURIFICATION

Biolytic Labs. also offers the innovative Dr. Oligo Processor for high throughput purification of oligonucleotides using Glen-Pak™ DNA Purification Cartridges: https://www.biolytic.com/p-6814-dr-oligo-processor-fully-automated.aspx.

See Also


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