Applied Biosystems Instruments (Catalog as PDF)

Sterling CE Phosphoramidites

Glen Research CE (β-cyanoethyl) Phosphoramidites are produced and packaged to ensure the highest performance on DNA synthesizers. Every Glen Research product is accompanied by a Certificate of Analysis and HPLC trace, showing the results of our QC testing. Every Glen Research monomer vial is specially cleaned to eliminate particulate contamination and tested to ensure a tight fit on synthesizers.
Item Catalog No. Pack Price ($)
10-1000-02 0.25g 12.50
10-1000-05 0.5g 25.00
10-1000-10 1.0g 50.00
10-1000-20 2.0g 100.00
10-1000-40 4.0g 200.00
10-1010-02 0.25g 12.50
10-1010-05 0.5g 25.00
10-1010-10 1.0g 50.00
10-1010-20 2.0g 100.00
10-1010-40 4.0g 200.00
10-1015-02 0.25g 12.50
10-1015-05 0.5g 25.00
10-1015-10 1.0g 50.00
10-1015-20 2.0g 100.00
10-1015-40 4.0g 200.00
10-1020-02 0.25g 12.50
10-1020-05 0.5g 25.00
10-1020-10 1.0g 50.00
10-1020-20 2.0g 100.00
10-1020-40 4.0g 200.00
10-1029-02 0.25g 12.50
10-1029-05 0.5g 25.00
10-1029-10 1.0g 50.00
10-1029-20 2.0g 100.00
10-1029-40 4.0g 200.00
10-1030-02 0.25g 12.50
10-1030-05 0.5g 25.00
10-1030-10 1.0g 50.00
10-1030-20 2.0g 100.00
10-1030-40 4.0g 200.00
dA
dA
dC
dC
Ac-dC
Ac-dC
dG
dG
dmf-dG
dmf-dG
dT
dT

QUALITY ASSURANCE

Every batch of these CE Phosphoramidites is tested as follows

  1. HPLC
    a) Identity is confirmed by comparison with a reference sample.
    b) Purity is determined by HPLC to be ≥98.0%.
  2. TLC
    Purity is verified by TLC.
  3. 31P NMR
    Purity is determined by 31P NMR to be ≥98%.
  4. Coupling Test
    Coupling efficiency is determined to be ≥99%.
  5. Solution Test
    A 0.1M solution is determined to be clear and free of particulate contamination.
  6. Loss on Drying
    Volatile contaminants are determined to be ≤2%.

Sterling Solvents/Reagents

All solvents and reagents are prepared to our exacting specifications to ensure the highest synthesis efficiency and are passed through a 0.2 micron filter during packaging to eliminate particulate contamination. Glen Research uses freshly sublimed 1H-tetrazole for premium performance on Applied Biosystems synthesizers.
Item Catalog No. Pack Price ($)
Activator
30-3100-45 1 45mL 40.00
30-3100-52 2 200mL 100.00
30-3100-57 3 450mL 200.00
30-3100-62 4 2000mL 760.00
Diluent
40-4050-45 60mL 12.00
40-4050-50 100mL 16.00
Cap Mix A
40-4110-45 1 45mL 16.00
40-4110-52 2 200mL 30.00
40-4110-57 3 450mL 72.00
40-4110-62 4 2000mL 325.00
Cap Mix B
40-4220-45 1 45mL 20.00
40-4220-52 2 200mL 40.00
40-4220-57 3 450mL 96.00
  (This Cap B solution is identical to the formulation produced by Applied Biosystems.)
40-4220-62 4 2000mL 425.00
Oxidizing Solution
40-4330-52 1,2 200mL 30.00
40-4330-57 3 450mL 72.00
40-4330-62 4 2000mL 325.00
Deblocking Mix
40-4140-57 1,2 450mL 36.00
40-4140-62 3,4 2000mL 144.00

ABI INSTRUMENTS

  1. 60mL septum-capped vials used on oldest ABI 380, 381 and 391 instruments. 200mL oxidizer and 450mL deblock screw-capped bottles also used on ABI 380, 381 and 391 instruments.
  2. Small screw-capped vials used on ABI 392 and 394 instruments.
  3. Larger screw-capped vials used on ABI 392. 394 and 3400 instruments.
  4. Large bottles used on ABI 3900 instruments.

ABBREVIATIONS

Ac2O = Acetic Anhydride
CE = Cyanoethyl
CPG = Controlled Pore Glass
DCA = Dichloacetic Acid
DCM = Dichloromethane
DMAP = Dimethylaminopyridine
dmf = dimethylformamidine
DMT = 4,4’-Dimethoxytrityl
I2 = Iodine
iPr = Isopropyl
MeIm = 1-Methylimidazole
µm = micromole(s)
nm = nanomole(s)
Pac = Phenoxyacetyl
PhOAc = Phenoxyacetyl
TCA = Trichloroacetic Acid
THF = Tetrahydrofuran

See Also

Sterling Supports

All Glen Research CPG supports use the standard long chain alkylamino (lcaa) linker but differ in the glass pore size, 500Å, 1000Å or 2000Å. The 500Å support is appropriate for shorter sequences, while the 1000Å supports perform better in the synthesis of longer (>30-mer) DNA sequences. The 2000Å support is best for very long (>150-mer) oligonucleotides. We have instituted an additional QC test for supports to show the length of oligo that can be prepared before a drop-off in coupling due to steric effects begins to occur. The drop-off point is recorded in the Certificate of Analysis. All Glen Research supports are fully end-capped to ensure that the CPG surface is totally inert, thereby avoiding the introduction of impurity sequences containing deletions at the 3'-terminus.
Catalog No. Catalog No. Catalog No. Catalog No. Catalog No. Catalog No. Catalog No. Pack Price($)
dA dC dG dT dA,dC,dG,dT
1 of
each base
Ac-dC dmf-dG    
500Å Columns
20-2100-42 20-2110-42 20-2120-42 20-2130-42 20-2140-42 20-2113-42 4x0.2µm 40.00
20-2100-41 20-2110-41 20-2120-41 20-2130-41 20-2140-41 20-2113-41 4x1.0µm 60.00
20-2100-13 20-2110-13 20-2120-13 20-2130-13 20-2113-13 1x10µm 100.00
1000Å Columns
20-2101-45 20-2111-45 20-2121-45 20-2131-45 20-2141-45 20-2115-45 20-2129-45 4x40nm 40.00
20-2101-42 20-2111-42 20-2121-42 20-2131-42 20-2141-42 20-2115-42 20-2129-42 4x0.2µm 40.00
20-2101-41 20-2111-41 20-2121-41 20-2131-41 20-2141-41 20-2115-41 20-2129-41 4x1.0µm 60.00
20-2101-13 20-2111-13 20-2121-13 20-2131-13 20-2115-13 20-2129-13 1x10µm 100.00
2000Å Columns
20-2102-42 20-2112-42 20-2122-42 20-2132-42 20-2142-42 4x0.2µm 40.00
Low Volume (LV) Polystyrene Columns
26-2100-45 26-2110-45 26-2120-45 26-2130-45 26-2140-45 4x40nm 48.00
26-2100-42 26-2110-42 26-2120-42 26-2130-42 26-2140-42 4x0.2µm 48.00
AB 3900 Polystyrene Columns
26-2600-65 26-2610-65 26-2630-65 26-2629-65 200x40nm 825.00
26-2600-62 26-2610-62 26-2630-62 26-2629-62 200x200nm 825.00
AB 3900 1000Å CPG Columns
20-2101-65 20-2131-65 20-2115-65 20-2129-65 200x40nm 600.00
20-2101-62 20-2131-62 20-2115-62 20-2129-62 200x200nm 650.00
20-2101-61 20-2131-61 20-2115-61 20-2129-61 200x1.0µm 875.00
500Å Bulk CPG
20-2000-01 20-2010-01 20-2020-01 20-2030-01 20-2013-01 0.1g 9.00
20-2000-02 20-2010-02 20-2020-02 20-2030-02 20-2013-02 0.25g 20.00
20-2000-10 20-2010-10 20-2020-10 20-2030-10 20-2013-10 1.0g 75.00
1000Å Bulk CPG
20-2001-01 20-2011-01 20-2021-01 20-2031-01 20-2015-01 20-2029-01 0.1g 9.00
20-2001-02 20-2011-02 20-2021-02 20-2031-02 20-2015-02 20-2029-02 0.25g 20.00
20-2001-10 20-2011-10 20-2021-10 20-2031-10 20-2015-10 20-2029-10 1.0g 75.00
2000Å Bulk CPG
20-2002-01 20-2012-01 20-2022-01 20-2032-01 0.1g 15.00
20-2002-02 20-2012-02 20-2022-02 20-2032-02 0.25g 30.00
20-2002-10 20-2012-10 20-2022-10 20-2032-10 1.0g 105.00
dA-CPG 500
dA-CPG 500
dC-CPG 500
dC-CPG 500
dG-CPG 500
dG-CPG 500
dT-CPG 500
dT-CPG 500
Ac-dC-CPG 500
Ac-dC-CPG 500
dA-CPG 1000
dA-CPG 1000
dC-CPG 1000
dC-CPG 1000
dG-CPG 1000
dG-CPG 1000
dT-CPG 1000
dT-CPG 1000
Ac-dC-CPG 1000
Ac-dC-CPG 1000
dmf-dG-CPG
dmf-dG-CPG
dA-CPG 2000
dA-CPG 2000
dC-CPG 2000
dC-CPG 2000
dG-CPG 2000
dG-CPG 2000
dT-CPG 2000
dT-CPG 2000

dA PS
dA PS
dC PS
dC PS
dT PS
dT PS

AB 3900 1000Å CPG Columns

Glen Research's AB 3900 1000Å CPG columns bring the lower cost of CPG to this platform while maintaining the high synthesis efficiency of 1000Å CPG. Our columns offer the following key attributes:

  • No need to change instrument settings
  • No need to change software parameters
  • Easier handling post -synthesis compared to PS
  • High quality 1000Å CPG for optimal synthesis results

BULK CPG LOADING

500Å supports 35-50µmoles/g
1000Å supports 25-40µmoles/g

See Also

Empty columns and frits

Item Catalog No. Pack Price ($)
20-0030-00 Pack of 10 60.00
20-0040-00 Pack of 10 300.00
20-0040-0F Pack of 20 30.00

TWIST is a trademark of Glen Research Corporation.

AB 3900 Polystyrene Modifier Columns

Some of our more popular minor base and modifier supports are available on polystyrene in columns fully compatible with the Applied Biosystems 3900 synthesizer. These include our popular Universal Support III, which will allow DNA, RNA or LNA oligos to be produced on the 3900 with ANY base at the 3' terminus. At the same time, we are offering 1 μmole columns of Universal Support III for the 3900 instrument. Structures and more complete descriptions are found in the relevant catalog sections for each item. AB 3900 columns can be prepared with virtually any of the CPG supports in this catalog. It is no longer necessary to adjust the flow using our AB 3900 CPG columns, as noted in the box to the right. Modified CPG columns are only available in 200 nmole size - simple add 'A' to the regular catalog number to order.
Item Catalog No. Pack Price ($)
AB 3900 Format
Universal Support III PS
26-5110-52 Pack of 10 100.00
  40 nmole columns (AB 3900 Format) 26-5110-55 Pack of 10 100.00
Glen UnySupport PS
26-5140-52 Pack of 10 100.00
  40 nmole columns 26-5140-55 Pack of 10 100.00
3'-Phosphate PS
26-2900-52 Pack of 10 150.00
  40 nmole columns 26-2900-55 Pack of 10 150.00
3'-PT-Amino-Modifier C6 PS
26-2956-52 Pack of 10 220.00
  40 nmole columns 26-2956-55 Pack of 10 220.00
3'-(6-FAM) PS
26-2961-52 Pack of 10 300.00
  40 nmole columns 26-2961-55 Pack of 10 300.00
3'-Dabcyl PS
26-5912-52 Pack of 10 300.00
  40 nmole columns 26-5912-55 Pack of 10 300.00
3'-TAMRA PS
26-5910-52 Pack of 10 300.00
  40 nmole columns 26-5910-55 Pack of 10 300.00
3'-BiotinTEG PS
26-2955-52 Pack of 10 300.00
  40 nmole columns 26-2955-55 Pack of 10 300.00
Universal Support III PS
Universal Support III PS
Glen UnySupport™ PS
Glen UnySupport™ PS
3'-Phosphate PS
3'-Phosphate PS
3'-PT-Amino-Modifier C6 PS
3'-PT-Amino-Modifier C6 PS
3'-(6-FAM) PS
3'-(6-FAM) PS
3'-Dabcyl PS
3'-Dabcyl PS
3'-TAMRA PS
3'-TAMRA PS
3'-BiotinTEG PS
3'-BiotinTEG PS

AB 3900 1000Å CPG Columns

Glen Research’s AB 3900 1000Å CPG columns bring the lower cost of CPG to this platform while maintaining the high synthesis efficiency of 1000Å CPG. Our columns offer the following key attributes:

  • No need to change instrument settings
  • No need to change software parameters
  • Easier handling post -synthesis compared to PS
  • High quality 1000Å CPG for optimal synthesis results

Intellectual Property

Glen UnySupport PS is covered by US Patent 7,202,264 owned by Ionis Pharmaceuticals, Inc..

Universal Support III PS is covered by US Patent Nos.: 6,770,754 and 7,491,817 and European Patent Nos.: 1404695 and 2248820.

See Also


Contact to place an order.
INTERNATIONAL USERS: Contact your distributor or order from USA
Contact to access more than 50 years of oligo expertise for help in designing oligos for your unique application.
Glen Research Corporation, Copyright © 2019