Modifiers (Catalog as PDF)

Terminus Modifiers

Glen Research 5'-Modifiers are designed for use in DNA synthesizers to functionalize the 5'-terminus of the target oligonucleotide. The 5'-Amino-Modifiers are available with a variety of chain lengths to fit exactly the desired application.

The DMS(O)MT-protected amino group is easier to deprotect compared to the MMT-protected one. The sulfoxy derivative survives conditions of oligonucleotide synthesis and can either be cleaved with standard deblock solution, or left intact for HPLC purification. At the same time, the DMS(O)MT group is fully compatible with cartridge purification. When detritylation on a cartridge is carried out, the DMS(O)MT+, which is more stable than MMT+, does not reattach itself to an amine. We now offer 5'-DMS(O)MT-Amino-Modifier C6 utilizing this new trityl based protecting group.

5'-Amino-Modifier TEG, a hydrophilic triethylene glycol ethylamine derivative, is 12 atoms in length and fully soluble in aqueous media.
Item Catalog No. Pack Price ($)
10-1923-90 100µm 50.00
10-1923-02 0.25g 175.00
10-1906-90 100µm 60.00
10-1906-02 0.25g 200.00
10-1916-90 100µm 30.00
10-1916-02 0.25g 100.00
10-1912-90 100µm 90.00
10-1912-02 0.25g 300.00
10-1905-90 100µm 60.00
10-1905-02 0.25g 200.00
10-1907-90 100µm 60.00
10-1907-02 0.25g 200.00
10-1917-90 100µm 115.00
10-1917-02 0.25g 500.00
5'-Amino-Modifier C3-TFA
5'-Amino-Modifier C3-TFA
5'-Amino-Modifier C6
5'-Amino-Modifier C6
5'-Amino-Modifier C6-TFA
5'-Amino-Modifier C6-TFA
5'-Amino-Modifier C12
5'-Amino-Modifier C12
5'-Amino-Modifier 5
5'-Amino-Modifier 5
5'-DMS(O)MT-Amino-Modifier C6
5'-DMS(O)MT-Amino-Modifier C6
5'-Amino-Modifier TEG CE-Phosphoramidite
5'-Amino-Modifier TEG CE-Phosphoramidite

ABBREVIATIONS

CE = Cyanoethyl
CNEt = Cyanoethyl
CPG = Controlled Pore Glass
DMT = 4,4'-Dimethoxytrityl
Fmoc = Fluorenylmethoxycarbonyl
iPr = Isopropyl
lcaa = long chain alkylamino
µm = micromole(s)
MMT = 4-Monomethoxytrityl
nm = nanomole(s)
T = Trityl
TBDMS = t-Butyl-dimethylsilyl
TFA = Trifluroacetyl

See Also

Terminus Modifiers (Part 2)

Our more recent 5'-amino modifiers, protected by a novel phthalic acid diamide (PDA) protecting group, are stable solids. In contrast to the TFA protected amino modifiers, which are viscous oils, the analogous PDA protected compounds are granular powders. This important property of these compounds allows straightforward handling, storage and aliquoting and leads to a significant increase in stability.

Deprotection with methylamine in gas phase or aqueous solution or AMA leads to fast and complete removal of the PDA protecting group. However, ammonium hydroxide will not drive the equilibrium reaction to completion and only partial deprotection occurs - overnight deprotection with ammonium hydroxide will yield around 80% active amine.

We are offering three PDA Amino-Modifiers:
  • 5'-Amino-Modifier C6-PDA
  • Hydrophobic 5'-Amino-Modifier C12-PDA
  • Hydrophilic 5'-Amino-Modifier-TEG-PDA
Item Catalog No. Pack Price ($)
10-1947-90 100µm 30.00
10-1947-02 0.25g 100.00
10-1948-90 100µm 65.00
10-1948-02 0.25g 240.00
10-1949-90 100µm 105.00
10-1949-02 0.25g 420.00
5'-Amino-Modifier C6-PDA
5'-Amino-Modifier C6-PDA
5'-Amino-Modifier C12-PDA
5'-Amino-Modifier C12-PDA
5'-Amino-Modifier TEG PDA
5'-Amino-Modifier TEG PDA

Intellectual Property

PDA amino-modifiers were developed by Stefan Pitsch and ReseaChem GmbH (S. Berger), Patent pending.

OTHER INSTRUMENT TYPES

All minor bases, RNA products and modifiers are packaged in septumcapped vials suitable for ABI and other instruments. If you would like another type of vial/column add the following to the end of the catalog number.

Monomers  

For Instrument type
Add
Expedite
E
Mermade
M

Columns  

For Instrument type
Add
Expedite
E
Applied Biosystems 3900
A
Mermade
M

Please inquire for availability of columns for other instrument types.

Terminus Modifiers (Part 3)

The disulfide thiol modifier may be used for introducing 3'- or 5'-thiol linkages. Dithiol Serinol, produced from lipoic acid and our patented serinol backbone, allows easy connection of multiple dithiol-labelled oligos to gold surfaces. 5'-Carboxy-Modifier C10 is a unique linker designed to be added at the terminus of an oligonucleotide synthesis. It generates an activated carboxylic acid N-hydroxysuccinimide (NHS) ester suitable for immediate conjugation on the synthesis column with molecules containing a primary amine, resulting in a stable amide linkage. An alternative carboxylate protecting group is the 2-chlorotrityl group, which is simply removed using the standard deblock cycle to generate a free carboxyl group on an otherwise fully protected oligonucleotide. The 2-chlorotrityl group is also removed during oligo deprotection with ammonium hydroxide or AMA and is incompatible with RP purification techniques. PC Amino-Modifier is a photocleavable C6 amino-modifier, part of our line of photocleavable (PC) modifiers. 5'-AminoOxy-Modifier 11 is based on a tetraethylene glycol linkage for improved solubility and for reducing the potential negative impact on hybridization of the oligo. The oxime formed from the reaction of alkyloxyamines with aldehydes creates a stable covalent bond. In comparison, the imine formed by the conjugation of primary amines with aldehydes is not stable to acidic or basic conditions and requires subsequent reduction with borohydride to form stable amine conjugates. 5'-Maleimide Modifier Phosphoramidite, developed at the University of Barcelona, incorporates a maleimide cycloadduct that is stable to ammonium hydroxide at room temperature. This phosphoramidite can be incorporated into DNA and RNA with both phosphate and phosphorothioate linkages. A retro-Diels-Alder reaction deprotects the maleimide immediately prior to conjugation.
Item Catalog No. Pack Price ($)
10-1926-90 100µm 60.00
10-1926-02 0.25g 200.00
10-1936-90 100µm 150.00
10-1936-02 0.25g 360.00
10-1991-95 50µm 120.00
10-1991-90 100µm 215.00
10-1991-02 0.25g 585.00
10-4906-90 100µm 135.00
10-4906-02 0.25g 395.00
10-1935-90 100µm 65.00
10-1935-02 0.25g 265.00
10-1945-90 100µm 95.00
10-1945-02 0.25g 330.00
10-1919-95 50µm 140.00
10-1919-90 100µm 265.00
10-1919-02 0.25g 895.00
10-1938-90 100µm 70.00
10-1938-02 0.25g 335.00
5'-Thiol-Modifier C6
5'-Thiol-Modifier C6
Thiol-Modifier C6 S-S
Thiol-Modifier C6 S-S
Dithiol Serinol
Dithiol Serinol
PC Amino-Modifier
PC Amino-Modifier
5'-Carboxy-Modifier C10
5'-Carboxy-Modifier C10
5'-Carboxy-Modifier C5
5'-Carboxy-Modifier C5
5'-Aminooxy-Modifier-11
5'-Aminooxy-Modifier-11
5'-Maleimide-Modifier
5'-Maleimide-Modifier

Intellectual Property

5'-Carboxy-Modifier C10 is offered for sale under license from TriLink BioTechnologies, Inc. It is intended for research and development purposes only, and may not be used for commercial, clinical, diagnostic or any other use. It is covered under US Patent No. 6,320,041.

5'-Maleimide Modifier Phosphoramidite is protected by a patent application and is offered by Glen Research under a license agreement from the University of Barcelona.

Glen Research offers PC Biotin, PC Amino-Modifier and PC Spacer products in association with AmberGen, Inc. and Link Technologies, Ltd. For a commercial application license, please contact AmberGen, Inc., +617-975-0680, http://www.ambergen.com/..

Serinol products are covered by US Patent No.: 8,394,948.

OTHER INSTRUMENT TYPES

All minor bases, RNA products and modifiers are packaged in septumcapped vials suitable for ABI and other instruments. If you would like another type of vial/column add the following to the end of the catalog number.

Monomers  

For Instrument type
Add
Expedite
E
Mermade
M

Columns  

For Instrument type
Add
Expedite
E
Applied Biosystems 3900
A
Mermade
M

Please inquire for availability of columns for other instrument types.

Sequence Modifiers

Sequence Modifiers are designed for use in automated synthesis. The carboxy-dT is hydrolyzed during deprotection and can be coupled directly to a molecule containing a primary amino group by a standard peptide coupling or via the intermediate N-hydroxysuccinimide (NHS) ester. Amino-Modifier dA, Amino-Modifier dC, N2-Amino-Modifier dG and both Amino-Modifier dT products can be added in place of a dA, dC, dG and dT residue, respectively, during oligonucleotide synthesis. Corresponding Amino-Modifier supports can replace their respective deoxynucleoside supports. After deprotection, the primary amine on the C6 analogues is separated from the oligonucleotide by a spacer arm with a total of 7 -10 atoms and can be labelled or attached to an enzyme. The C2 analogue is more suitable for the attachment of molecules designed to react with the oligonucleotide.
Item Catalog No. Pack Price ($)
10-1089-90 100µm 205.00
10-1089-02 0.25g 455.00
10-1019-90 100µm 225.00
10-1019-02 0.25g 450.00
10-1529-95 50µm 240.00
10-1529-90 100µm 480.00
10-1529-02 0.25g 1100.00
10-1035-90 100µm 180.00
10-1035-02 0.25g 360.00
10-1037-90 100µm 180.00
10-1037-02 0.25g 360.00
10-1037-05 0.5g 720.00
10-1039-90 100µm 180.00
10-1039-02 0.25g 360.00
10-1039-05 0.5g 720.00
Amino-Modifier C6 dA
Amino-Modifier C6 dA
Amino-Modifier C6 dC
Amino-Modifier C6 dC
N2-Amino-Modifier C6 dG
N2-Amino-Modifier C6 dG
Carboxy-dT
Carboxy-dT
Amino-Modifier C2 dT
Amino-Modifier C2 dT
Amino-Modifier C6 dT
Amino-Modifier C6 dT

See Also

Sequence Modifiers (Part 2)

Our repertoire of NHS ester derivatives has been expanded to include the NHS-Carboxy-dT-CE Phosphoramidite. By making a dT analog of the Carboxy-Modifier C10, it is possible to label one or multiple sites within an oligonucleotide. This opens up the possibility to label any number of different dyes or molecules within an oligonucleotide when the phosphoramidite is unavailable. Doing so is straightforward and may be done manually off the synthesizer or even in a fully-automated manner on the DNA synthesizer.

We have never found conditions which allow the TFA group to be removed from an amino-modifier while the oligonucleotide remains attached to the support. We are able to solve this problem by using a 9-fluorenylmethoxycarbonyl (Fmoc) protecting group. The Fmoc group is removed using a two step procedure, the first to remove the cyanoethyl protection groups and flush the formed acrylonitrile from the synthesis column using 1% diisopropylamine in acetonitrile, and the second to remove the Fmoc group using 10% piperidine in DMF. The amino group so formed on the column can be reacted with a variety of activated esters. We offer Fmoc-Amino-Modifier C6 dT Phosphoramidite as a nucleosidic option and Amino-Modifier Serinol Phosphoramidite as a non-nucleosidic alternative. We also offer S-Bz-Thiol-Modifier C6-dT to join the ranks of thiol-modifiers for oligonucleotide synthesis. Thiol-Modifier C6-dT can be added as usual at the desired locations within a sequence.
Item Catalog No. Pack Price ($)
10-1535-90 100µm 210.00
10-1535-02 0.25g 550.00
10-1536-90 100µm 180.00
10-1536-02 0.25g 360.00
10-1538-95 50µm 130.00
10-1538-90 100µm 245.00
10-1538-02 0.25g 550.00
10-1997-95 50µm 125.00
10-1997-90 100µm 225.00
10-1997-02 0.25g 595.00
NHS-Carboxy-dT
NHS-Carboxy-dT
Fmoc Amino-Modifier C6 dT
Fmoc Amino-Modifier C6 dT
S-Bz-Thiol-Modifier C6-dT
S-Bz-Thiol-Modifier C6-dT
Amino-Modifier Serinol
Amino-Modifier Serinol

Intellectual Property

Serinol products are covered by US Patent No.: 8,394,948.

OTHER INSTRUMENT TYPES

All minor bases, RNA products and modifiers are packaged in septumcapped vials suitable for ABI and other instruments. If you would like another type of vial/column add the following to the end of the catalog number.

Monomers  

For Instrument type
Add
Expedite
E
Mermade
M

Columns  

For Instrument type
Add
Expedite
E
Applied Biosystems 3900
A
Mermade
M

Please inquire for availability of columns for other instrument types.

See Also

3'-Modifiers

3'-Amino-Modifier CPGs, containing amino groups protected with the base-labile Fmoc group, are designed to functionalize the 3'-terminus of the target oligonucleotide by the introduction of a primary amine. In an alternative approach, the nitrogen destined to become the 3'-amino group is included in a phthalimide (PT) group which is attached to the support through an amide group attached to the aromatic ring. This simple linkage is very stable to all conditions of oligonucleotide synthesis and contains no chiral center. Using an extended ammonium hydroxide treatment (55°C for 17 hours), the cleavage of the amine from the phthalimide is accomplished along with the deprotection of the oligonucleotide. ABI-style columns are supplied unless otherwise requested.
Item Catalog No. Pack Price ($)
20-2958-01 0.1g 95.00
20-2958-10 1.0g 675.00
  1 µmole columns 20-2958-41 Pack of 4 140.00
  0.2 µmole columns 20-2958-42 Pack of 4 85.00
  10 µmole column (ABI) 20-2958-13 Pack of 1 250.00
  15 µmole column (Expedite) 20-2958-14 Pack of 1 375.00
20-2997-01 0.1g 95.00
20-2997-10 1.0g 675.00
  0.2 µmole columns 20-2997-42 Pack of 4 85.00
  0.2 µmole columns 20-2997-42 Pack of 4 85.00
  1 µmole columns 20-2997-41 Pack of 4 140.00
  10 µmole column (ABI) 20-2997-13 Pack of 1 250.00
  15 µmole column (Expedite) 20-2997-14 Pack of 1 375.00
20-2954-01 0.1g 95.00
20-2954-10 1.0g 675.00
  1 µmole columns 20-2954-41 Pack of 4 140.00
  0.2 µmole columns 20-2954-42 Pack of 4 85.00
  10 µmole column (ABI) 20-2954-13 Pack of 1 250.00
  15 µmole column (Expedite) 20-2954-14 Pack of 1 375.00
20-2956-01 0.1g 95.00
20-2956-10 1.0g 675.00
  1 µmole columns 20-2956-41 Pack of 4 140.00
  0.2 µmole columns 20-2956-42 Pack of 4 85.00
  10 µmole column (ABI) 20-2956-13 Pack of 1 250.00
  15 µmole column (Expedite) 20-2956-14 Pack of 1 375.00
26-2956-01 0.1g 125.00
26-2956-10 1.0g 1025.00
  200 nmole columns 26-2956-52 Pack of 10 220.00
  40 nmole columns 26-2956-55 Pack of 10 220.00
3'-Amino-Modifier C7 CPG 1000
3'-Amino-Modifier C7 CPG 1000
3'-Amino-Modifier Serinol CPG
3'-Amino-Modifier Serinol CPG
3'-PT-Amino-Modifier C3 CPG
3'-PT-Amino-Modifier C3 CPG
3'-PT-Amino-Modifier C6 PS
3'-PT-Amino-Modifier C6 PS
3'-PT-Amino-Modifier C6 CPG
3'-PT-Amino-Modifier C6 CPG

Intellectual Property

Serinol products are covered by US Patent No.: 8,394,948.

3'-Modifiers (Part 2)

The 3'-Thiol-Modifier S-S CPG supports are used to introduce 3'-thiol linkages with three and six atom spacers into oligonucleotides. 3'-Dithiol Serinol CPG is used to introduce a dithiol group at the 3'-terminus. In conjunction with Dithiol Serinol Phosphoramidite, it is simple to produce oligonucleotides with multiple thiol groups at the 3' terminus, which is ideal for conjugation to gold surfaces. With Glyceryl CPG the 3'-terminus of an oligonucleotide is readily oxidized by sodium periodate to form a 3'-phosphoglycaldehyde. The aldehyde may be further oxidized to the corresponding carboxylic acid. Either the aldehyde or the carboxylate may be used for subsequent conjugation to amine-containing products.
Item Catalog No. Pack Price ($)
20-2933-01 0.1g 85.00
20-2933-10 1.0g 600.00
  1 µmole columns 20-2933-41 Pack of 4 125.00
  0.2 µmole columns 20-2933-42 Pack of 4 75.00
  10 µmole column (ABI) 20-2933-13 Pack of 1 225.00
  15 µmole column (Expedite) 20-2933-14 Pack of 1 350.00
20-2938-01 0.1g 85.00
20-2938-10 1.0g 600.00
  0.2 µmole columns 20-2938-42 Pack of 4 75.00
  1 µmole columns 20-2938-41 Pack of 4 125.00
  10 µmole column (ABI) 20-2938-13 Pack of 1 225.00
  15 µmole column (Expedite) 20-2938-14 Pack of 1 350.00
20-2991-01 0.1g 120.00
20-2991-10 1.0g 995.00
  1 µmole columns 20-2991-41 Pack of 4 200.00
  0.2 µmole columns 20-2991-42 Pack of 4 120.00
  10 µmole column (ABI) 20-2991-13 Pack of 1 300.00
  15 µmole column (Expedite) 20-2991-14 Pack of 1 450.00
20-2902-01 0.1g 85.00
20-2902-10 1.0g 600.00
  1 µmole columns 20-2902-41 Pack of 4 125.00
  0.2 µmole columns 20-2902-42 Pack of 4 75.00
  10 µmole column (ABI) 20-2902-13 Pack of 1 225.00
  15 µmole column (Expedite) 20-2902-14 Pack of 1 350.00
3'-Thiol-Modifier C3 S-S CPG
3'-Thiol-Modifier C3 S-S CPG
3'-Thiol-Modifier 6 S-S CPG
3'-Thiol-Modifier 6 S-S CPG
3'-Dithiol Serinol CPG
3'-Dithiol Serinol CPG
3'-Glyceryl CPG
3'-Glyceryl CPG

Intellectual Property

3'-Thiol-Modifier 6 S-S CPG was developed by, and is sold under agreement from Berry & Associates.

Serinol products are covered by US Patent No.: 8,394,948.

OTHER INSTRUMENT TYPES

All minor bases, RNA products and modifiers are packaged in septumcapped vials suitable for ABI and other instruments. If you would like another type of vial/column add the following to the end of the catalog number.

Monomers  

For Instrument type
Add
Expedite
E
Mermade
M

Columns  

For Instrument type
Add
Expedite
E
Applied Biosystems 3900
A
Mermade
M

Please inquire for availability of columns for other instrument types.

See Also

3'-Modifiers (Part 3)

3'-Amino-Modifier C6 dC CPG and 3'-Amino-Modifier C6 dT CPG replace a dC and T, respectively, at the 3'-terminus. These products allow convenient labelling at the 3' without blocking the terminus from desired enzymatic activity.
Item Catalog No. Pack Price ($)
20-2019-01 0.1g 120.00
20-2019-10 1.0g 995.00
  1 µmole columns 20-2019-41 Pack of 4 200.00
  0.2 µmole columns 20-2019-42 Pack of 4 120.00
  10 µmole column (ABI) 20-2019-13 Pack of 1 300.00
  15 µmole column (Expedite) 20-2019-14 Pack of 1 450.00
20-2039-01 0.1g 96.00
20-2039-10 1.0g 800.00
  1 µmole columns 20-2039-41 Pack of 4 160.00
  0.2 µmole columns 20-2039-42 Pack of 4 96.00
  10 µmole column (ABI) 20-2039-13 Pack of 1 240.00
  15 µmole column (Expedite) 20-2039-14 Pack of 1 360.00
3'-Amino-Modifier C6 dC CPG
3'-Amino-Modifier C6 dC CPG
3'-Amino-Modifier C6 dT CPG
3'-Amino-Modifier C6 dT CPG

Chemical Phosphorylation

Chemical Phosphorylation Reagent is most commonly used to phosphorylate the 5'-terminus of an oligonucleotide. Although this product is also successful in 3'-phosphorylation, 3'-Phosphate CPG allows direct preparation of oligonucleotides with a 3'-phosphate group. Chemical Phosphorylation Reagent II contains a DMT group on a side chain which is stable to base cleavage and can be left on the oligonucleotide for use in RP purification. The DMT group is later removed with aqueous acid and the side chain is eliminated after brief treatment with aqueous ammonium hydroxide to yield the 5'-phosphate.1 Solid CPR II is similar in performance to CPR II but it is easier to prepare aliquots since it is a powder. Many researchers treat synthesis supports with a hindered base (e.g., diethylamine, diisopropylethylamine, or DBU) post-synthesis to eliminate and remove the cyanoethyl phosphate groups. In this way, the acrylonitrile formed in situ is removed from the support and is not available to alkylate dT residues at the N3 position in the oligos. Since the sulfonylethyl group in 3'-Phosphate CPG is also susceptible to β-elimination leading to oligo cleavage, this technique is not compatible with 3'-phosphate CPG. Using CPR II CPG, which is base labile but does not support β-elimination, the cyanoethyl groups can be removed from the oligo prior to cleavage and base deprotection. ABI-style vials and columns are supplied unless otherwise requested.
Item Catalog No. Pack Price ($)
10-1900-90 100µm 50.00
10-1900-02 0.25g 160.00
20-2900-01 0.1g 70.00
20-2900-10 1.0g 480.00
  1 µmole columns 20-2900-41 Pack of 4 100.00
  0.2 µmole columns 20-2900-42 Pack of 4 60.00
  10 µmole column (ABI) 20-2900-13 Pack of 1 180.00
  15 µmole column (Expedite) 20-2900-14 Pack of 1 280.00
26-2900-01 0.1g 75.00
26-2900-10 1.0g 510.00
  200 nmole columns 26-2900-52 Pack of 10 150.00
  40 nmole columns 26-2900-55 Pack of 10 150.00
25-2900-01 0.1g 85.00
25-2900-10 1.0g 600.00
25-2900-46 4x2.5µm 120.00
10-1901-90 100µm 60.00
10-1901-02 0.25g 200.00
10-1902-90 100µm 60.00
10-1902-02 0.25g 200.00
20-2903-01 0.1g 70.00
20-2903-10 1.0g 480.00
  0.2 µmole columns 20-2903-42 Pack of 4 60.00
  1 µmole columns 20-2903-41 Pack of 4 100.00
  10 µmole column (ABI) 20-2903-13 Pack of 1 180.00
  15 µmole column (Expedite) 20-2903-14 Pack of 1 280.00
Chemical Phosphorylation Reagent
Chemical Phosphorylation Reagent
3'-Phosphate CPG
3'-Phosphate CPG
3'-Phosphate PS
3'-Phosphate PS
3'-Phosphate CPG (High Load)
3'-Phosphate CPG (High Load)
Chemical Phosphorylation Reagent II
Chemical Phosphorylation Reagent II
Solid Chemical Phosphorylation Reagent II
Solid Chemical Phosphorylation Reagent II
3'-CPR II CPG
3'-CPR II CPG

Intellectual Property

Solid Chemical Phosphorylation Reagent II and related supports are covered by European Patent: EP0816368.

Reference(s)

  1. A. Guzaev, , A. Azhayev, and H. Lonnberg, Tetrahedron, 1995, 51, 9375-9384.

See Also

Aldehyde Modification

Aldehyde modifiers would be attractive electrophilic substitutions in oligonucleotides since they are able to react with amino groups to form a Schiff's base, with hydrazino groups to form hydrazones, and with semicarbazides to form semi-carbazones. The Schiff's base is unstable and must be reduced with sodium borohydride to form a stable linkage but hydrazones and semicarbazides are very stable linkages.

Our collaboration with ELITechGroup, formerly Epoch Biosciences, has allowed us to offer 5'-Aldehyde-Modifier C2 Phosphoramidite. The acetal protecting group is sufficiently hydrophobic for use in RP HPLC and cartridge purification and is readily removed after oligonucleotide synthesis under standard oligonucleotide detritylation conditions with 80% acetic acid / 20% water or 2% aqueous trifluoroacetic acid during cartridge purification.

A formylindole nucleoside analogue has been used to introduce aldehyde groups within an oligonucleotide or at the 5' terminus. This product has no protecting group on the aldehyde, which means that deprotection of the modified oligonucleotide can be done without changing preferred conditions.
Item Catalog No. Pack Price ($)
10-1933-90 100µm 85.00
10-1933-02 0.25g 325.00
10-1934-90 100µm 85.00
10-1934-02 0.25g 325.00
5'-Aldehyde-Modifier C2
5'-Aldehyde-Modifier C2
5-Formylindole
5-Formylindole

Intellectual Property

This Product is for research purposes only, and may not be used for commercial, clinical, diagnostic or any other use. This Product is subject to proprietary rights of ELITechGroup and are made and sold under license from ELITechGroup. There is no implied license for commercial use with respect to these Products and a license must be obtained directly from ELITechGroup with respect to any proposed commercial use of these Products. “Commercial use” includes but is not limited to the sale, lease, license or other transfer of the Product or any material derived or produced from it, the sale, lease, license or other grant of rights to use the Product or any material derived or produced from it, or the use of the Product to perform services for a fee for third parties (including contract research).

A simple agreement must be signed before end-users and custom oligo services may purchase these products for use as defined above. http://www.glenresearch.com/
Reference/AP-dCEmail.pdf

Spacer Modifiers

The spacer phosphoramidites C3, 9, C12 and 18 are used to insert a spacer arm in an oligonucleotide. The compounds may be added in multiple additions when a longer spacer is required. 3'-Spacer C3 CPG may also act as a blocker of exonuclease and polymerase activity at the 3'-terminus. dSpacer is used to introduce a stable abasic site within an oligonucleotide. PC Spacer is a photocleavable C3 spacer modifier, part of our line of photocleavable (PC) modifiers.
Item Catalog No. Pack Price ($)
10-1909-90 100µm 75.00
10-1909-02 0.25g 240.00
10-1913-90 100µm 75.00
10-1913-02 0.25g 240.00
10-1914-90 100µm 85.00
10-1914-02 0.25g 295.00
10-1918-90 100µm 95.00
10-1918-02 0.25g 240.00
10-1928-90 100µm 95.00
10-1928-02 0.25g 240.00
20-2913-01 0.1g 70.00
20-2913-10 1.0g 480.00
  1 µmole columns 20-2913-41 Pack of 4 100.00
  0.2 µmole columns 20-2913-42 Pack of 4 60.00
  10 µmole column (ABI) 20-2913-13 Pack of 1 180.00
  15 µmole column (Expedite) 20-2913-14 Pack of 1 280.00
10-4913-90 100µm 135.00
10-4913-02 0.25g 395.00
Spacer 9
Spacer 9
Spacer C3
Spacer C3
dSpacer CE
dSpacer CE
Spacer 18
Spacer 18
Spacer C12 CE
Spacer C12 CE
3'-Spacer C3 CPG
3'-Spacer C3 CPG
PC Spacer
PC Spacer

Intellectual Property

Glen Research offers PC Biotin, PC Amino-Modifier and PC Spacer products in association with AmberGen, Inc. and Link Technologies, Ltd. For a commercial application license, please contact AmberGen, Inc., +617-975-0680, http://www.ambergen.com/..

OTHER INSTRUMENT TYPES

All minor bases, RNA products and modifiers are packaged in septumcapped vials suitable for ABI and other instruments. If you would like another type of vial/column add the following to the end of the catalog number.

Monomers  

For Instrument type
Add
Expedite
E
Mermade
M

Columns  

For Instrument type
Add
Expedite
E
Applied Biosystems 3900
A
Mermade
M

Please inquire for availability of columns for other instrument types.

See Also

Dendrimers

Dendrimers are discrete, highly branched, monodispersed polymers that possess patterns reminiscent of the branching of trees. Plain and mixed oligonucleotide dendrimers can be synthesized using novel doubling and trebling phosphoramidite synthons.1,2 Dendrimers offer the following advantages. Incorporation of label using g-32P-ATP and polynucleotide kinase increases in proportion to the number of 5'-ends. Fluorescent signal also increases in proportion to the number of 5'-ends, if spacers are incorporated between the labels and the ends of the branches. When using a dendrimeric oligonucleotide as a PCR primer, the strand bearing the dendrimer is resistant to degradation by T7 Gene 6 exonuclease making it easy to convert the double-stranded product of the PCR to a multiply labelled, single-stranded probe. Enhanced stability of DNA dendrimers makes them useful as building blocks for the 'bottom up' approach to nano-assembly. These features also suggest applications in DNA chip technology when higher temperatures are required, for example, to melt secondary structure in the target.
Item Catalog No. Pack Price ($)
10-1920-90 100µm 150.00
10-1920-02 0.25g 240.00
10-1981-90 100µm 105.00
10-1981-02 0.25g 250.00
10-1922-90 100µm 180.00
10-1922-02 0.25g 300.00
10-1925-90 100µm 200.00
10-1925-02 0.25g 300.00
Symmetric Doubler
Symmetric Doubler
Asymmetric Doubler (Lev)
Asymmetric Doubler (Lev)
Trebler
Trebler
Long Trebler
Long Trebler

Intellectual Property

These products are supplied under license from Oxford University Innovation Limited.

Reference(s)

  1. M.S. Shchepinov, I.A. Udalova, A.J. Bridgman, and E.M. Southern, Nucleic Acids Res, 1997, 25, 4447- 4454.
  2. M.S. Shchepinov, K.U. Mir, J.K. Elder, M.D. Frank-Kamenetskii, and E.M. Southern, Nucleic Acids Res, 1999, 27, 3035-41.

OTHER INSTRUMENT TYPES

All minor bases, RNA products and modifiers are packaged in septumcapped vials suitable for ABI and other instruments. If you would like another type of vial/column add the following to the end of the catalog number.

Monomers  

For Instrument type
Add
Expedite
E
Mermade
M

Columns  

For Instrument type
Add
Expedite
E
Applied Biosystems 3900
A
Mermade
M

Please inquire for availability of columns for other instrument types.

Branching Phosphoramidite

A branching monomer is required to construct comb-like oligonucleotide probes. The developers of the comb system from Chiron Corporation evaluated1 several protecting groups for the branch point and chose levulinyl (LEV), which is specifically removed using a reagent containing hydrazine hydrate, acetic acid and pyridine.
Item Catalog No. Pack Price ($)
10-1018-90 100µm 205.00
10-1018-02 0.25g 505.00
5-Me-dC Brancher
5-Me-dC Brancher

Reference(s)

  1. T. Horn, C.A. Chang, and M.S. Urdea, Nucleic Acids Res, 1997, 25, 4842-4849.

Photocleavable Modifiers

PC Biotin Phosphoramidite can be used to prepare 5'-biotinylated oligonucleotides suitable for capture by streptavidin in a mode similar to our popular 5' Biotin Phosphoramidite. Amino- and thiol-modified oligonucleotides have proven to be very useful for the attachment of a variety of haptens and fluorophores, as well as for the tethering of the oligonucleotides to a diversity of beads and surfaces. PC Amino-Modifier Phosphoramidite is used to prepare 5'-amino-modified oligonucleotides suitable for subsequent photocleavage. PC Spacer Phosphoramidite can be used as an intermediary to attach any modification reagent, available as a phosphoramidite, to the terminus of oligonucleotides. After photocleavage, a 5'-phosphate is generated on the DNA, rendering it suitable for further biological transformations, such as gene construction and cloning after ligation.

A versatile photocleavable DNA building block has been described by researchers in Washington University, Missouri and used in phototriggered hybridization.1 This reagent has also been used in the design of multifunctional DNA and RNA conjugates2 for the in vitro selection of new molecules catalyzing biomolecular reactions. Researchers at Bruker Daltonik in Germany have also developed genoSNIP, a method for single-nucleotide polymorphism (SNP) genotyping by MALDI-TOF mass spectrometry.3 This method uses size reduction of primer extension products by incorporation of the photocleavable linker for phototriggering strand breaks near to the 3' end of the extension primer. PC Linker can be incorporated into oligonucleotides at any position by standard automated DNA synthesis methodology. PC Linker Phosphoramidite has the added advantage in that photocleavage results in monophosphate fragments at both the 3'- and 5'-termini of the oligonucleotide fragments.
Item Catalog No. Pack Price ($)
10-4950-95 50µm 145.00
10-4950-90 100µm 280.00
10-4950-02 0.25g 675.00
10-4906-90 100µm 135.00
10-4906-02 0.25g 395.00
10-4913-90 100µm 135.00
10-4913-02 0.25g 395.00
10-4920-90 100µm 255.00
10-4920-02 0.25g 795.00
PC Biotin
PC Biotin
PC Amino-Modifier
PC Amino-Modifier
PC Linker
PC Linker
PC Spacer
PC Spacer

Intellectual Property

Glen Research offers PC Biotin, PC Amino-Modifier and PC Spacer products in association with AmberGen, Inc. and Link Technologies, Ltd. For a commercial application license, please contact AmberGen, Inc., +617-975-0680, http://www.ambergen.com/..

Reference(s)

  1. P. Ordoukhanian and J-S. Taylor, J. Am. Chem. Soc., 117, 9570-9571, 1995.
  2. (a) F. Hausch and A. Jäschke, Nucleic Acids Research, 2000, 28, e35.
  3. (b) F. Hausch and A. Jäschke, Tetrahedron, 2001, 57, 1261-1268.
  4. T. Wenzel, T. Elssner, K. Fahr, J. Bimmler, S. Richter, I. Thomas, and M. Kostrzewa, Nucleosides, Nucleotides & Nucleic Acids, 2003, 22, 1579-1581.

OTHER INSTRUMENT TYPES

All minor bases, RNA products and modifiers are packaged in septumcapped vials suitable for ABI and other instruments. If you would like another type of vial/column add the following to the end of the catalog number.

Monomers  

For Instrument type
Add
Expedite
E
Mermade
M

Columns  

For Instrument type
Add
Expedite
E
Applied Biosystems 3900
A
Mermade
M

Please inquire for availability of columns for other instrument types.

See Also

Conjugation using Click Chemistry

The copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) reaction between azides and alkynes to form 1,2,3-triazoles, as reported1 by Sharpless, was found to be so exquisitely regioselective and efficient at even the most mild conditions that Sharpless coined the term 'Click Chemistry' to describe it. The use of this method for DNA modification has been somewhat delayed by the fact that copper ions damage DNA, typically yielding strand breaks.2 As these problems have now been overcome by the use of copper(I)-stabilizing ligands (e.g., tris(benzyltriazolylmethyl)amine, TBTA3), Carell et al. and Seela et al. discovered that the CuAAC reaction can be used to functionalize alkyne-modified DNA nucleobases with extremely high efficiency.4

Oligonucleotides bearing a single nucleosidic alkyne group can be prepared using a C8-Alkyne-dC or dT-CE Phosphoramidite. Purified oligonucleotides are usually modified with 2-5 equivalents of the corresponding marker-azide (e.g., fluorescent-dye azides). After the addition of precomplexed Cu(I), complete conversion to the labelled oligo is observed in a time span between 30 min and 4 hours. After a simple precipitation step, labelled oligonucleotides can be recovered in near quantitative yields. Using a combination of C8-Alkyne, C8-TIPS-Alkyne and C8-TMS-Alkyne, it is possible to label oligonucleotides in up to three separate click reactions. The alkyne groups on the last two monomers are protected, respectively, with triisopropylsilyl (TIPS) and trimethylsilyl (TMS) protecting groups.5,6 The first click reaction on solid phase on a C8-Alkyne yields the singly modified oligonucleotide with full retention of the TIPS and/or TMS protecting group. For double click, a C8-TIPS-Alkyne is used as the second nucleoside and the TIPS protecting group is cleaved with tetrabutylammonium fluoride (TBAF) without causing any damage to the DNA. The second click reaction in solution yields the doubly modified oligonucleotide in excellent yield. For the introduction of three different labels, all three nucleosides are introduced into oligonucleotides. The first click reaction is performed directly on the resin. The singly modified oligonucleotide is subsequently cleaved from the support with concomitant cleavage of the TMS group and retention of the TIPS protecting group. The second click reaction is performed in solution. Precipitation of the doubly modified oligonucleotide, cleavage of the TIPS group with TBAF, and a subsequent third click reaction in solution furnishes the desired triply modified oligonucleotide in excellent overall yield.
Item Catalog No. Pack Price ($)
10-1540-95 50µm 165.00
10-1540-90 100µm 315.00
10-1540-02 0.25g 900.00
10-1541-95 50µm 295.00
10-1541-90 100µm 575.00
10-1541-02 0.25g 1275.00
10-1542-95 50µm 270.00
10-1542-90 100µm 525.00
10-1542-02 0.25g 1275.00
10-1543-95 50µm 225.00
10-1543-90 100µm 435.00
10-1543-02 0.25g 1125.00
C8-Alkyne-dT
C8-Alkyne-dT
C8-TIPS-Alkyne-dC
C8-TIPS-Alkyne-dC
C8-TMS-Alkyne-dC
C8-TMS-Alkyne-dC
C8-Alkyne-dC
C8-Alkyne-dC

Intellectual Property

All products of baseclick are patent protected and available in collaboration with baseclick.

Baseclick GmbH has filed the following patent applications:

1. WO2006/117161, New labelling strategies for the sensitive detection of analytes.

2. WO2008/952775, Click Chemistry for the production of reporter molecules.

Baseclick GmbH holds a worldwide license for the research market of the "Click Chemistry" patent from "The Scripps Research Institute":

3. WO03/101972, Copper-catalysed ligation of azides and acetylenes.

OTHER INSTRUMENT TYPES

All minor bases, RNA products and modifiers are packaged in septumcapped vials suitable for ABI and other instruments. If you would like another type of vial/column add the following to the end of the catalog number.

Monomers  

For Instrument type
Add
Expedite
E
Mermade
M

Columns  

For Instrument type
Add
Expedite
E
Applied Biosystems 3900
A
Mermade
M

Please inquire for availability of columns for other instrument types.

Conjugation using Click Chemistry (Part 2)

5-Ethynyl-dU offers convenient click conjugation with an azide to generate a label rigidly attached to one of the oligonucleotide bases. 5-Ethynyl-dU is subject to base-catalyzed hydration during cleavage and deprotection, especially when using a strong base or heat. Hydration of an ethynyl group forms a methyl ketone which subsequently blocks potential click reactions. Mild deprotection conditions are necessary when using 5-Ethynyl-dU-CE Phosphoramidite to prevent this side reaction. TIPS-5-Ethynyl-dU-CE Phosphoramidite, containing a protected alkyne, offers broader compatibility with oligonucleotide synthesis and deprotection. Protecting the 5-ethynyl group with a triisopropylsilyl (TIPS) protecting group prevents acid or base catalyzed hydration during oligonucleotide synthesis and workup. A quick treatment with TBAF removes the TIPS protecting group.
Item Catalog No. Pack Price ($)
10-1544-95 50µm 220.00
10-1544-90 100µm 425.00
10-1544-02 0.25g 1020.00
10-1545-95 50µm 205.00
10-1545-90 100µm 395.00
10-1545-02 0.25g 1050.00
10-1554-95 50µm 130.00
10-1554-90 100µm 245.00
10-1554-02 0.25g 775.00
10-1555-95 50µm 195.00
10-1555-90 100µm 370.00
10-1555-02 0.25g 975.00
50-1004-92 25 µmole 50.00
50-1004-90 100 µmole 180.00
50-1002-11 10x1ml Discontinued
  Click-Solution (DMSO/t-BuOH) has been discontinued.
C8-TIPS-Alkyne-dT
C8-TIPS-Alkyne-dT
C8-TMS-Alkyne-dT
C8-TMS-Alkyne-dT
5-Ethynyl-dU
5-Ethynyl-dU
TIPS-5-Ethynyl-dU
TIPS-5-Ethynyl-dU
THPTA Ligand
THPTA Ligand

Intellectual Property

baseclick GmbH has been granted the following patents (1-3) besides its further patent applications (4-5).

1. WO  2006/117161  (New  labelling  strategies  for  the  sensitive detection of analytes)

2. WO 2008/952775 (Click chemistry for the production of reporter molecules)

3. WO 2010/115957 (Click Chemistry on heterogeneous catalysts)

4. PCT/EP 2013/064610 (Anandamide-modified nucleic molecules)

5. PCT/EP 2015/056007 (Self-assembly of DNA Origami: a diagnostic tool)

baseclick GmbH holds a worldwide exclusive license for granted patent application

WO 03/101972 (Copper-catalysed ligation of azides and acetylenes  for  the  nucleic  acid field) in the area of diagnostics and research. As Glen Research and baseclick are partners, Glen Research is now able to help in sublicensing this outstanding technology.

Reference(s)

  1. C.W. Tornoe, C. Christensen, M. Meldal, J. Org. Chem. 2002, 67, 3057-3064; V. V. Rostovtsev, L. G. Green, V. V. Fokin, K. B. Sharpless, Angew. Chem. 2002, 114, 2708-2711; Angew. Chem. Int. Ed. 2002, 41, 2596-2599.
  2. C. J. Burrows, J. G. Muller, Chem. Rev. 1998, 98, 1109 – 1151.
  3. T. R. Chan, R. Hilgraf, K. B. Sharpless, V. V. Fokin, Org. Lett. 2004, 6, 2853 – 2855.
  4. J. Gierlich, G. A. Burley, P. M. E. Gramlich, D. M. Hammond, T. Carell, Org. Lett. 2006, 8, 3639-3642. F. Seela, V. R. Sirivolu, Chem. Biodiversity 2006, 3, 509-514.
  5. P. M. E. Gramlich, S. Warncke, J. Gierlich, T. Carell, Angew. Chem. 2008, 120, 3491–3493; Angew. Chem. Int. Ed. 2008, 47, 3442– 3444.
  6. P. M. E. Gramlich, C. T. Wirges, A. Manetto, T. Carell, Angew. Chem. Int. Ed. 2008, 47, 8350-8358.

Conjugation using Click Chemistry (Part 3)

Oligonucleotides prepared using 5'-Hexynyl Phosphoramidite are stable to standard deprotection conditions and exhibit a slightly increased retention time on RP HPLC. Azides are not compatible with oligonucleotide synthesis using phosphoramidites so a post-synthesis reaction is required. Azidobutyrate NHS Ester is used1 for azido-modification of amines at either the 3'-end or the 5'-end of an oligo and it can even be used for internal modification on an Amino-Modifier-C6 dX residue within the sequence. Specific to the 5'-terminus, 5'-Bromohexyl Phosphoramidite is added in the last cycle. This modifier can then be easily transformed into a 5'-azido group by displacement of bromide using sodium azide.2 Alkyne NHS ester allows the functionalization of an amino moiety in a variety of molecules, including DNA and RNA oligonucleotides as well as peptides or proteins. We also offer two products for use in Click Chemistry based upon our 1,3-diol product portfolio with the serinol backbone - a phosphoramidite for adding an alkyne group at the 5' terminus or within the sequence, and a synthesis support for labelling the 3' terminus of oligonucleotides with an alkyne group.
Item Catalog No. Pack Price ($)
10-1908-90 100µm 60.00
10-1908-02 0.25g 200.00
50-1904-23 2.3mg 60.00
50-1904-24 23mg 300.00
  (Dissolve 2.3mg in 60µL of DMSO)
10-1946-90 100µm 60.00
10-1946-02 0.25g 200.00
50-1905-23 2.3mg 60.00
50-1905-24 23mg 300.00
  (Dissolve 2.3mg in 60µL of DMSO)
10-1992-95 50µm 100.00
10-1992-90 100µm 185.00
10-1992-02 0.25g 575.00
20-2992-01 0.1g 105.00
20-2992-10 1.0g 800.00
  0.2 µmole columns 20-2992-42 Pack of 4 100.00
  1 µmole columns 20-2992-41 Pack of 4 175.00
  10 µmole column (ABI) 20-2992-13 Pack of 1 260.00
  15 µmole column (Expedite) 20-2992-14 Pack of 1 390.00
5'-Hexynyl
5'-Hexynyl
Azidobutyrate NHS Ester
Azidobutyrate NHS Ester
5'-Bromohexyl
5'-Bromohexyl
Alkyne-NHS Ester
Alkyne-NHS Ester
Alkyne-Modifier Serinol
Alkyne-Modifier Serinol
3'-Alkyne-Modifier Serinol CPG
3'-Alkyne-Modifier Serinol CPG

Intellectual Property

Serinol products are covered by US Patent No.: 8,394,948.

Reference(s)

  1. R. Kumar, et al., Journal of the American Chemical Society, 2007, 129, 6859-6864.
  2. J. Lietard, A. Meyer, J.J. Vasseur, and F. Morvan, Tetrahedron Letters, 2007, 48, 8795-8798.

See Also

Conjugation using Click Chemistry (Part 4)

1-Ethynyl-dSpacer CE Phosphoramidite can be used in any position within an oligonucleotide while still retaining the high efficiency of click chemistry. The modifier is efficiently incorporated into oligonucleotides using standard phosphoramidite chemistry, is stable to common deprotection conditions, and is compatible with Glen-Pak™ purification. 1-Ethynyl-dSpacer generates a substituted 1,2,3-triazole pseudo-nucleobase after click chemistry conjugation with an azide The 1-ethynyl-dSpacer modification exhibits similar duplex stability to the standard dSpacer (10-1914) and destabilizes the duplex when internally incorporated. Upon cycloaddition, the duplex stability is moderated by the resulting structure of the modification. Simple 1,2,3-triazoles were destabilizing, as were modifications that incorporated TEG linkers (6-FAM-TEG and Amino-TEG). Modifications that incorporated aromatic functional groups restored duplex stability to varying degrees with coumarin and psoralen significantly restoring stability.

A 5'-iodo-modified oligonucleotide (prepared using 5'-Iodo-dT) can be quantitatively converted to the corresponding 5'-azide
Item Catalog No. Pack Price ($)
10-1910-95 50µm 180.00
10-1910-90 100µm 340.00
10-1910-02 0.25g 1250.00
10-1931-90 100µm 85.00
10-1931-02 0.25g 295.00
1-Ethynyl-dSpacer CE
1-Ethynyl-dSpacer CE
5'-I-dT
5'-I-dT

STABILITY NOTES

Oligonucleotides containing a 5'-iodo group are prepared conventionally with the exception that deprotection is carried out in ammonium hydroxide at room temperature for 24 hours. Under these conditions, degradation of the iodo group was less than 2%.

OTHER INSTRUMENT TYPES

All minor bases, RNA products and modifiers are packaged in septumcapped vials suitable for ABI and other instruments. If you would like another type of vial/column add the following to the end of the catalog number.

Monomers  

For Instrument type
Add
Expedite
E
Mermade
M

Columns  

For Instrument type
Add
Expedite
E
Applied Biosystems 3900
A
Mermade
M

Please inquire for availability of columns for other instrument types.

See Also

OLIGO-CLICK KITS

Oligo-Click Kits contain an air-stable, insoluble Cu(I) source in pellet form in a pre-loaded and ready-to-use vial. Within the kit, the TBTA ligand is replaced by an activator which is compatible with both aqueous and organic solvents. This innovative combination of catalyst and ligand/activator results in a much easier labelling work-flow of only three simple steps. The preparation of the oligonucleotide labelling via CuAAC now requires only a minimal hands-on time of a few minutes or even less and can be carried out in air without any additional precautions. Glen Research is offering the following kits in collaboration with baseclick GmbH.
  • Oligo-Kit M Reload: This kit has sufficient reagents for conjugating up to nine alkyne-containing oligonucleotides on a 100 nmole scale or a single oligonucleotide on a 1 µmole scale. The user must supply the azide and a solvent such as DMSO for dissolving the azide.

  • Oligo-Kit M Biotin, Oligo-Kit M Fluorescein and Oligo-Kit M TAMRA: Each kit has sufficient reagents for conjugating up to seven alkyne-containing oligonucleotides on a 100 nmole scale or a single oligonucleotide on a 1 µmole scale. Each kit contains all of the ingredients necessary, including the azide and DMSO solvent.
  • Item Catalog No. Pack Price ($)
    50-2100-01 1 Kit 120.00
    50-2101-01 1 Kit 200.00
    50-2102-01 1 Kit 240.00
    50-2103-01 1 Kit 270.00

See Also

Copper-free Click Chemistry

At Glen Research, our goal was to offer a copper-free click phosphoramidite reagent with the following properties:
  • Simple to use
  • Stable in solution on the synthesizer
  • Stable to ammonium hydroxide and AMA
  • Excellent click performance in 17 hours or less at room temperature

From the variety of cyclooctyne-based copper-free click reagents so far described, we have chosen to offer compounds based on a dibenzo-cyclooctyne (DBCO) structure. We are offering 5'-DBCO-TEG Phosphoramidite for preparing oligos with a 5'-DBCO modification and DBCO-dT-CE Phosphoramidite for inserting a DBCO group at any position within the oligonucleotide. In addition, we offer a further DBCO phosphoramidite - DBCO-Serinol Phosphoramidite. Using our proprietary serinol backbone as a non-nucleosidic spacer allows the DBCO group to be placed at any location within a sequence with multiple additions clearly possible. DBCO-sulfo-NHS Ester is also offered for post-synthesis conjugation reactions. DBCO-modified oligos may be conjugated with azides in organic solvents, such as DMSO, or aqeous buffers. Depending on the azide used, the reaction will go to completion in 4-17 hours at room temperature. Simple desalting on a Glen Gel-Pak™ leads to a product with virtually quantitative conjugation efficiency.

Note: We now recommend that synthesis of oligos containing DBCO-dT be completed using 0.5 M CSO in anhydrous acetonitrile (40-4632-xx).  Acceptable results can be achieved with iodine oxidation if DBCO-dT is subjected to no more than 8-10 cycles.

Item Catalog No. Pack Price ($)
10-1941-95 50µm 125.00
10-1941-90 100µm 230.00
10-1941-02 0.25g 775.00
10-1539-95 50µm 250.00
10-1539-90 100µm 485.00
10-1539-02 0.25g 975.00
50-1941-23 5.2mg 60.00
50-1941-24 52mg 300.00
  (Dissolve 5.2mg in 60µL water or DMSO)
10-1998-95 50µm 180.00
10-1998-90 100µm 340.00
10-1998-02 0.25g 895.00
5'-DBCO-TEG
5'-DBCO-TEG
DBCO-dT
DBCO-dT
DBCO-sulfo-NHS Ester
DBCO-sulfo-NHS Ester
DBCO-Serinol
DBCO-Serinol

OTHER INSTRUMENT TYPES

All minor bases, RNA products and modifiers are packaged in septumcapped vials suitable for ABI and other instruments. If you would like another type of vial/column add the following to the end of the catalog number.

Monomers  

For Instrument type
Add
Expedite
E
Mermade
M

Columns  

For Instrument type
Add
Expedite
E
Applied Biosystems 3900
A
Mermade
M

Please inquire for availability of columns for other instrument types.

See Also

Conjugation using Click Chemistry (Part 6)

Glen Research is offering first our most popular labels for general interest and, subsequently, we will add azide products that are not compatible with phosphoramidite chemistry.

Biotin is still our most commonly used label and biotinTEG, with its hydrophilic triethylene glycol spacer, is the most popular biotin product. Desthiobiotin is a biotin analogue that is well captured by streptavidin but the captured product can be easily released by applying a biotin solution to the streptavidin beads. 6-FAM is our most popular fluorescein derivative and we offer azides of both 6-FAM and pivaloyl-protected 6-FAM for situations where subsequent reactions require the 6-FAM to be protected. In both 6-FAM products, the hydrophilic TEG spacer is again used. The azides are offered in 25 and 100 µmole packs for convenient oligonucleotide labelling.

7-Hydroxycoumarin, also known as umbelliferone, is a highly fluorescent, pH-sensitive fluorophore that emits in the blue region of the spectrum. However, its fluorescence is strongly quenched if the hydroxyl is alkylated or phosphorylated, making it useful in high-throughput screening for phosphatases and lipases. Interestingly, it was found that the 3-azido derivative is also highly quenched but, upon reaction with an alkyne in the presence of copper to form the triazole, the fluorescence is restored.1 The clicked coumarin emits at a lambda max of 480 nm and absorbs at 358 nm.

HEX and TET are two of our most popular fluorescein-based dyes for labelling oligonucleotides. We are happy to offer 6-HEX and 6-TET Azides for use in click conjugations.
Item Catalog No. Pack Price ($)
50-2000-92 25 µmole 150.00
50-2000-90 100 µmole 450.00
50-2001-92 25 µmole 135.00
50-2001-90 100 µmole 400.00
50-2002-92 25 µmole 230.00
50-2002-90 100 µmole 690.00
50-2003-92 25 µmole 180.00
50-2003-90 100 µmole 540.00
50-2004-92 25 µmole 115.00
50-2004-90 100 µmole 350.00
50-2005-92 25 µmole 150.00
50-2005-90 100 µmole 450.00
50-2006-92 25 µmole 150.00
50-2006-90 100 µmole 450.00
BiotinTEG Azide
BiotinTEG Azide
DesthiobiotinTEG Azide
DesthiobiotinTEG Azide
Dipivaloyl 6-FAM-TEG Azide
Dipivaloyl 6-FAM-TEG Azide
6-FAM-TEG Azide
6-FAM-TEG Azide
Coumarin Azide
Coumarin Azide
6-HEX Azide
6-HEX Azide
6-TET Azide
6-TET Azide

Reference(s)

  1. J. Gierlich, G.A. Burley, P.M. Gramlich, D.M. Hammond, and T. Carell, Org Lett, 2006, 8, 3639-42.

OTHER INSTRUMENT TYPES

All minor bases, RNA products and modifiers are packaged in septumcapped vials suitable for ABI and other instruments. If you would like another type of vial/column add the following to the end of the catalog number.

Monomers  

For Instrument type
Add
Expedite
E
Mermade
M

Columns  

For Instrument type
Add
Expedite
E
Applied Biosystems 3900
A
Mermade
M

Please inquire for availability of columns for other instrument types.

Conjugation using Click Chemistry (Part 7)

Two nitroxide spin labels, TEMPO Azide and TEMPO-TEG Azide, for site directed spin labelling (SDSL) are now offered.

Click Chemistry with psoralen azide and one of our many nucleosidic and non-nucleosidic alkyne derivatives has the potential to generate a variety of practical cross-linkers. The well known reversible cross-linking behavior of psoralen with an adjacent thymidine residue could be very useful.

To better address applications in near-infrared (NIR) imaging, Glen Research is offering a water soluble Disulfo-Cyanine 7 azide that can be easily conjugated to DNA and RNA through standard click chemistry. This long wavelength dye offers the benefits of improved solubility, reduced aggregation, and improved stability in the near-infrared spectrum along with the convenience of click chemistry.
Item Catalog No. Pack Price ($)
50-2007-92 25 µmole 115.00
50-2007-90 100 µmole 350.00
50-2008-92 25 µmole 135.00
50-2008-90 100 µmole 400.00
50-2009-92 25 µmole 115.00
50-2009-90 100 µmole 350.00
50-2010-92 25 µmole 325.00
50-2010-90 100 µmole 975.00
TEMPO Azide
TEMPO Azide
TEMPO-TEG Azide
TEMPO-TEG Azide
Psoralen Azide
Psoralen Azide
Disulfo-Cyanine 7 Azide
Disulfo-Cyanine 7 Azide

Contact to place an order.
INTERNATIONAL USERS: Contact your distributor or order from USA
Contact to access more than 50 years of oligo expertise for help in designing oligos for your unique application.
Glen Research Corporation, Copyright © 2019