Intro ABI Expedite Biosearch 8000 Cyclone MerMade PolyPlex Beckman Oligo Pharmacia UltraMild Supports Reagents

5'-3' Synthesis Me-Phosphonamidite Me-Phosphoramidite H-Phosphonate Sulfurization ODM

Duplex Stabilization PCR/Sequencing Structural

UltraMILD Terminus Sequence 3'-Modifiers CPR Spacers Dendrimers Photocleavable OPeC

Dabcyl Biotin Fluorescein Rhodamine Cyanine Acridine DNP Cholesterol Psoralen EDTA

TOM RNA Amidites TOM RNA Supports inor Base TOM RNA RNA Amidites RNA Supports Minor Base RNA 2'-OMe-RNA 2'-OMe-Supports Minor 2'-OMe 2'-F-RNA 2'-Se-Me-RNA

ABI Expedite Biosearch 8000 Cyclone Mermade Polyplex Beckman Pharmacia/Amersham UltraMild Alternatives H-Phosphonates Sulfurizing Complete List

NAIM Triphosphates Purification Miscellaneous Physical Properties Index General ;Information

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STERLING CE PHOSPHORAMIDITES

Glen Research CE (ß-cyanoethyl) Phosphoramidites are produced and packaged to ensure the highest performance on DNA synthesizers. Every Glen Research product is accompanied by a Certificate of Analysis and HPLC trace, showing the results of our QC testing. Every Glen Research monomer vial is specially cleaned to eliminate particulate contamination.

Item	Catalog No.	Pack	Price ($)
dA-CE Phosphoramidite	10-1000-B2	0.25g	12.50
	10-1000-B5	0.5g	25.00
	10-1000-1B	1.0g	50.00
dC-CE Phosphoramidite	10-1010-B2	0.25g	12.50
	10-1010-B5	0.5g	25.00
	10-1010-1B	1.0g	50.00
Ac-dC-CE Phosphoramidite	10-1015-B2	0.25g	12.50
	10-1015-B5	0.5g	25.00
	10-1015-1B	1.0g	50.00
dG-CE Phosphoramidite	10-1020-B2	0.25g	12.50
	10-1020-B5	0.5g	25.00
	10-1020-1B	1.0g	50.00
dmf-dG-CE Phosphoramidite	10-1029-B2	0.25g	12.50
	10-1029-B5	0.5g	25.00
	10-1029-1B	1.0g	50.00
dT-CE Phosphoramidite	10-1030-B2	0.25g	12.50
	10-1030-B5	0.5g	25.00
	10-1030-1B	1.0g	50.00

QUALITY ASSURANCE

Every batch of these CE Phosphoramidites is tested as follows

HPLC
a) Identity is confirmed by comparison with a reference sample.
b) Purity is determined by HPLC to be ≥98.0%.
TLC
Purity is verified by TLC.
31P NMR
Purity is determined by 31P NMR to be ≥98%.
Coupling Test
Coupling efficiency is determined to be ≥99%.
Solution Test
A 0.1M solution is determined to be clear and free of particulate contamination.
Loss on Drying
Volatile contaminants are determined to be ≤2%.

STERLING SOLVENTS/REAGENTS

All solvents and reagents are prepared to our exacting specifications to ensure the highest synthesis efficiency and are passed through a 0.2 micron filter during packaging to eliminate particulate contamination. Glen Research uses freshly sublimed 1H-tetrazole for premium performance on DNA synthesizers.

Item	Catalog No.	Pack	Price ($)
Activator
0.45M Tetrazole in Acetonitrile	30-3100-52	200mL	100.00
Diluent
Acetonitrile, anhydrous	40-4050-45	60mL	12.00
	40-4050-50	100mL	16.00
Cap Mix A
THF/Lutidine/Ac₂O (8:1:1)	40-4010-52	200mL	30.00
Cap Mix B
10% MeIm in THF	40-4120-52	200mL	30.00
16% MeIm in THF	40-4220-52	200mL	40.00
Oxidizing Solution
3% I₂in THF/Pyridine/H₂O This item has been discontinued. We suggest 40-4330-xx as the alternative.	40-4430-52	200mL
0.02M Iodine in Tetrahydrofuran/Pyridine/Water	40-4330-52	200mL	30.00
Deblocking Mix
3% TCA/DCM	40-4140-57	450mL	36.00

ABBREVIATIONS

Ac₂O = Acetic Anhydride
CE = Cyanoethyl
CPG = Controlled Pore Glass
DCM = Dichloromethane
I₂= Iodine
MeIm = 1-Methylimidazole
THF = Tetrahydrofuran
TCA = Trichloroacetic Acid

STERLING SUPPORTS

Oligo 1000 instruments use unique columns available from Beckman Instruments and we recommend these columns. However, Applied Biosystems columns, see Pages 7-8, can be used on Oligo 1000 instruments with the use of an adapter available from Beckman, Part Number 607824.

http://www.glenres.com/Catalog/beckman.html

Please contact Glen Research if you have any questions or comments!