Phosphonoacetate (PACE) modified oligonucleotides show great potential as biological modifiers in a wide variety of research applications. They have been shown to be active in siRNA duplexes and accelerate the initial rate of cleavage by RNase H-1 when incorporated with phosphorothioates. However, the most interesting observation to date is that they exhibit an unprecedented enhancement in penetration of cultured cells. Oligonucleotides containing this modification are quite simple to prepare using PACE monomers from Glen Research.

Several of our customers have requested a new backbone based on a 1,3-diol that would overcome any technical or IP issues surrounding our current products. Our response is a new line of products based on the serinol backbone. These products have been developed in close collaboration between Glen Research and Nelson Biotechnologies. At this early stage, we are offering phosphoramidites and supports for Amino-Modification, and Biotin and Fluorescein labelling. The biotin structures are protected to avoid any branching from the biotin molecule itself. These products all exhibit the high purity and performance that is standard for all Glen Research products.

In association with TagCyx Biotechnologies, we have added to our product range the amidites of dDs, ds and dPa, the elements for a new unnatural base pair system based on complementarity using specific hydrophobicity and shape. These monomers are used for DNA template synthesis. We are now supplying Biotin PaTP and sTP for their incorporation by T7 RNA polymerase. Biotin PaTP can be site-specifically incorporated into RNA, opposite dDs at a desired position in DNA templates and the fluorescent s base can be site-specifically incorporated into RNA opposite dPa in DNA templates, both using the in vitro T7 transcription system.

	3'-DTPA CPG
3'-Thiol-Modifier C6 S-S CPG
5'-Dichloro-dimethoxy-Fluorescein (JOE™)

Technical Briefs

Deprotection-Volume 1-Deprotect to Completion

Glen-Pak purification of 6-FAM oligos

New application for 5-Me-iso-dC and iso-dG

New application for 5'-OMe-dT

RECENT HIGHLIGHTS

Click Chemistry

Thiophosphoramidites

5'-CholesterylTEG Phosphoramidite

Solid Chemical Phosphorylation Reagent

Formylindole

8-D-dG-CE Phosphoramidite

Universal Support III

A Comparative Study of Universal Supports

Pyrrolidine CE Phosphoramidite

Pyrene-dU, Perylene-dU

Fmoc-Amino-Modifier C6-dT

Large Scale Synthesis

Long Trebler

New Amino-Modifier

NHS-Carboxy-dT

AP-dC (G-clamp) UPDATE

Oligonucleotide Caps

PREVIOUS Technical Briefs

RNA

Activators

Examining the ribonuclease H primer grip of HIV-1 reverse transcriptase by charge neutralization of RNA/DNA hybrids
Chandravanu Dash, Brian J. Scarth, Christopher Badorrek, Matthias Götte, and Stuart F. J. Le Grice
Nucleic Acids Res.,Oct 2008;
10.1093/nar/gkn678

conferences and posters