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Catalog Number: 13-1031-xx
Description: ATC Trimer Phosphoramidite
| 5'-Dimethoxytrityl-N6-benzoyl-2'-deoxyAdenosine-3'->5'-o-chlorophenyl-phosphoryl- 2'-deoxyThymidine-3'->5'-o-chlorophenyl-phosphoryl-N4-benzoyl- 2'-deoxyCytidine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C85H87Cl2N12O21P3 | M.W.: 1774.5 | |
Diluent: Anhydrous Acetonitrile/Dichloromethane 1:3 (v/v) |
| Coupling:15 minute coupling time recommended. |
| Deprotection: 30% NH4OH for 17 hours at room temperature followed by an additional 4 hours at 55°C. |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 2-3 days |
| Catalog Information | Material Safety Data Sheet |
Literature Highlights
| Glen Report 16.2: TRIMER (CODON) PHOSPHORAMIDITES SIMPLIFY LIBRARY PREPARATION |
| Glen Report 18.1: Trimer Phosphoramidites - Tools for fine-tuning protein function |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | 0.1M Dil. (mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 13-1031-90 | 100µmoles | .177grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 13-1031-95 | 50µmoles | .089grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| Expedite | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 13-1031-90 | 100µmoles | .177grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 13-1031-95 | 50µmoles | .089grams | .75 | .07 | 8.6 | 5.38 | 3.91 | .54 | |
| Beckman | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 13-1031-90 | 100µmoles | .177grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 13-1031-95 | 50µmoles | .089grams | .75 | .07 | 10.2 | 6.38 | 4.64 | ||
11/14/2011 | http://www.glenres.com/ProductFiles/13-1031.html