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Catalog Number: 10-3430-xx
Description: 2'-F-U-CE Phosphoramidite
| 5'-Dimethoxytrityl-deoxyUridine, 2'-fluoro-3'-[(2-cyanoethyl)- (N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C39H46FN4O8P | M.W.: 748.79 | F.W.: 308.16 |
Diluent: Anhydrous Acetonitrile |
| Coupling: 3 minute coupling time recommended. |
| Deprotection: Ammonium Hydroxide for 17 hours at 55°C or 30% Ammonia Hydroxide/40% Methylamine 1:1 (AMA) for 2 hours at Room Temperature. (Note - heating in AMA will lead to some degradation of the 2'-Fluoro nucleotides.) |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 24 hours |
| Please Note: A license may be required from Isis Pharmaceuticals, Inc. to incorporate 2'-fluoro modified nucleosides into oligonucleotides as claimed in US Patent Numbers 5,670,633; 6,005,087; 6,531,584 and foreign equivalents. |
| Catalog Information | Material Safety Data Sheet |
Literature Highlights
| Glen Report 10.1: MORE NOVEL MONOMERS |
| Glen Report 17.1: 2'-FLUORO-RNA Monomers |
| Glen Report 21.1: TECHNICAL BRIEF Ð LNA VS 2Õ-F-RNA |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | 0.1M Dil. (mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-3430-02 | 0.25grams | .25grams | 3.34 | 98 | 58.8 | 36.75 | 26.73 | 19.6 | 4.9 |
| 10-3430-05 | 0.5grams | .5grams | 6.68 | 209.33 | 125.6 | 78.5 | 57.09 | 41.87 | 10.47 |
| Expedite | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-3430-02 | 0.25grams | .25grams | 4.98 | .07 | 93.2 | 58.25 | 42.36 | 5.83 | |
| 10-3430-05 | 0.5grams | .5grams | 9.97 | .07 | 193 | 120.63 | 87.73 | 12.06 | |
| Beckman | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-3430-02 | 0.25grams | .25grams | 4.98 | .07 | 94.8 | 59.25 | 43.09 | ||
| 10-3430-05 | 0.5grams | .5grams | 9.97 | .07 | 194.6 | 121.63 | 88.45 | ||
11/04/2011 | http://www.glenres.com/ProductFiles/10-3430.html