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Catalog Number: 10-1975-xx
Description: Cholesteryl-TEG Phosphoramidite
| 1-Dimethoxytrityloxy-3-O-(N-cholesteryl-3-aminopropyl)-triethyleneglycol- glyceryl-2-O-(2-cyanoethyl)-(N,N,-diisopropyl)-phosphoramidite | ||
| Formula: C70H106N3O11P | M.W.: 1196.60 | F.W.: 755.97 |
Diluent: 10% THF in Anhydrous Acetonitrile Add fresh diluent to product vial to recommended concentration and swirl vial occasionally over several minutes until product is completely dissolved. (Some oils may require between 5 and 10 minutes.) Use care to maintain anhydrous conditions. In case of transfer to alternate vial type, ensure recipient vial has been pre-dried. For more information, see http://www.glenres.com/Technical/TB_ABITransfer.pdf. |
| Coupling: 15 minute coupling time. To maintain label yield, carry out the synthesis DMT-on. |
| Deprotection: No changes needed from standard method recommended by synthesizer manufacturer. To maintain label yield, remove the 5'-DMT after ammonium hydroxide deprotection. |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 2-3 days |
| Catalog Information | Material Safety Data Sheet |
Literature Highlights
| Glen Report 6.2: RESEARCH REVIEW - ANTISENSE RNA |
| Glen Report 6.1: NEW LABELLING REAGENTS |
| Glen Report 20.1: New Product |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | 0.1M Dil. (mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1975-02 | 0.25grams | .25grams | 2.09 | 56.33 | 33.8 | 21.13 | 15.36 | 11.27 | 2.82 |
| 10-1975-90 | 100µmoles | .12grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1975-95 | 50µmoles | .06grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| Expedite | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1975-02 | 0.25grams | .25grams | 3.12 | .067 | 56 | 35 | 25.45 | 3.5 | |
| 10-1975-90 | 100µmoles | .12grams | 1.5 | .067 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-1975-95 | 50µmoles | .06grams | .75 | .067 | 8.6 | 5.38 | 3.91 | .54 | |
| Beckman | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1975-02 | 0.25grams | .25grams | 3.12 | .067 | 57.6 | 36 | 26.18 | ||
| 10-1975-90 | 100µmoles | .12grams | 1.5 | .067 | 25.2 | 15.75 | 11.45 | ||
| 10-1975-95 | 50µmoles | .06grams | .75 | .067 | 10.2 | 6.38 | 4.64 | ||
05/03/2010 | http://www.glenres.com/ProductFiles/10-1975.html