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Catalog Number: 10-1170-xx
Description: dT-PACE Phosphoramidite
| 5'-Dimethoxytrityl-2'-deoxyThymidine, 3'-O-(N,N-diisopropylamino)-phosphinyl-1,1-dimethyl-2-cyanoethyl acetate | ||
| Formula: C44H55N4O9P | M.W.: 814.90 | F.W.: 345.22 |
Diluent: Anhydrous Acetonitrile |
| Coupling: 33 minutes with 1H-Tetrazole Activator. Synthesis: Oxidize before capping. Use either low water, low iodine oxidizer (Cat.# 40-4032-xx) or 10-camphorsulfonyloxaziridine (CSO) in acetonitrile. When CSO is used, extend the oxidation time to 3 minutes. Use 0.5M CSO when oxidizing mixed PACE/phosphodiester linkages. When oxidizing only PACE linkages, 0.1 M CSO can be used. For Cap Mix B, use 6.5% DMAP in THF (Cat.# 40-4020-xx). An example of a PACE cycle for AB synthesizers can be seen at:http://www.glenresearch.com/Technical/PACE_cycle.pdf |
| Deprotection: Pretreat synthesis column with 1.5% DBU in anhydrous acetonitrile for 60 minutes (syringe method). Wash with acetonitrile and dry with argon. Deprotect with 40% Methylamine for 20 minutes at 55°C. |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 2-3 days |
| Please Note: This product is covered by US Patents 6,693,187 and 7,067,641, patents pending and foreign counterparts owned by Lievre Cornu. Purchase of all or any of these products includes a limited license to use the product solely in the manufacture of oligonucleotides for RESEARCH USE ONLY and its use is not authorized nor intended for diagnostic or therapeutic use. |
| Please see Terms and Conditions of Use |
| Catalog Information | Material Safety Data Sheet |
Literature Highlights
| Glen Report 20.2: Phosphonoacetate (PACE) Oligonucleotides |
| Glen Report 20.2: Synthesis, Cleavage and Deprotection of PACE Oligonucleotides |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | 0.1M Dil. (mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1170-02 | 0.25grams | .25grams | 3.07 | 89 | 53.4 | 33.38 | 24.27 | 17.8 | 4.45 |
| 10-1170-05 | 0.5grams | .5grams | 6.14 | 191.33 | 114.8 | 71.75 | 52.18 | 38.27 | 9.57 |
| 10-1170-10 | 1.0gram | 1grams | 12.27 | 395.67 | 237.4 | 148.38 | 107.91 | 79.13 | 19.78 |
| Expedite | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1170-02 | 0.25grams | .25grams | 4.58 | .07 | 85.2 | 53.25 | 38.73 | 5.33 | |
| 10-1170-05 | 0.5grams | .5grams | 9.16 | .07 | 176.8 | 110.5 | 80.36 | 11.05 | |
| 10-1170-10 | 1.0gram | 1grams | 18.32 | .07 | 360 | 225 | 163.64 | 22.5 | |
| Beckman | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1170-02 | 0.25grams | .25grams | 4.58 | .07 | 86.8 | 54.25 | 39.45 | ||
| 10-1170-05 | 0.5grams | .5grams | 9.16 | .07 | 178.4 | 111.5 | 81.09 | ||
| 10-1170-10 | 1.0gram | 1grams | 18.32 | .07 | 361.6 | 226 | 164.36 | ||
11/04/2011 | http://www.glenres.com/ProductFiles/10-1170.html