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Catalog Number: 10-1100-xx
Description: dA-Me Phosphonamidite
| 5'-Dimethoxytrityl-N-benzoyl-2'-deoxyAdenosine,3'-[(methyl)- (N,N-diisopropyl)]-phosphonamidite | ||
| Formula: C45H51N6O6P | M.W.: 802.91 | F.W.: 311.24 |
Diluent: Anhydrous Acetonitrile |
| Coupling: 6 minutes. Note to prevent degradation of the methyl phosphonate linkage, low-water content oxidizer (40-4032) and DMAP for Cap B (40-4020) are recommended. |
| Deprotection: See Technical Bulletin. Deprotection Detail |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
| Stability in Solution: 24 hours |
| For a review of the synthesis and deprotection of Me-phosphonate oligonucleotides, see Protocols for Oligonucleotides and Analogs, Chapter 7, page 143, Editor:S.Agrawal, Publisher: Humana Press, 1993. Another Paper on Deprotection, see Hogrefe et. al., Nucleic Acids Res. , 1993, 21, 2031-2039. |
| Catalog Information | Material Safety Data Sheet | Technical Bulletin |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | 0.1M Dil. (mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1100-02 | 0.25grams | .25grams | 3.11 | 90.33 | 54.2 | 33.88 | 24.64 | 18.07 | 4.52 |
| 10-1100-05 | 0.5grams | .5grams | 6.23 | 194.33 | 116.6 | 72.88 | 53 | 38.87 | 9.72 |
| Expedite | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1100-02 | 0.25grams | .25grams | 5 | .062 | 93.6 | 58.5 | 42.55 | 5.85 | |
| 10-1100-05 | 0.5grams | .5grams | 10 | .062 | 193.6 | 121 | 88 | 12.1 | |
| Beckman | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1100-02 | 0.25grams | .25grams | 5 | .062 | 95.2 | 59.5 | 43.27 | ||
| 10-1100-05 | 0.5grams | .5grams | 10 | .062 | 195.2 | 122 | 88.73 | ||
03/19/2010 | http://www.glenres.com/ProductFiles/10-1100.html