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Catalog Number: 10-1093-xx
Description: 5-Hydroxymethyl-dU-CE Phosphoramidite
| 5'-Dimethoxytrityl-5-acetoxymethyl-2'-deoxyUridine,3'-[(2-cyanoethyl)- (N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C42H51N4O10P | M.W.: 802.86 | F.W.: 320.19 |
Diluent: Anhydrous Acetonitrile |
| Coupling: No changes needed from standard method recommended by synthesizer manufacturer. |
| Deprotection: Synthesize using acetyl-protected dC (10-1015-xx) and deprotect in 30% Ammonium Hydroxide/40% Methylamine 1:1 (AMA) at 65°C for 10 minutes OR deprotect in Ammonium Hydroxide for 2 hours at room temperature to remove the acetyl protecting group and complete the deprotection under temperatures and times suitable to remove the protecting groups of the nucleobases. |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
| Stability in Solution: 2-3 days |
| Catalog Information | Material Safety Data Sheet |
Literature Highlights
| Glen Report 12.1: MORE NOVEL MONOMERS |
| Glen Report 23.2: Technical Brief - Deprotection of hmdU |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | 0.1M Dil. (mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1093-02 | 0.25grams | .25grams | 3.11 | 90.33 | 54.2 | 33.88 | 24.64 | 18.07 | 4.52 |
| 10-1093-90 | 100µmoles | .08grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| Expedite | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1093-02 | 0.25grams | .25grams | 4.65 | .07 | 86.6 | 54.13 | 39.36 | 5.41 | |
| 10-1093-90 | 100µmoles | .08grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| Beckman | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1093-02 | 0.25grams | .25grams | 4.65 | .07 | 88.2 | 55.13 | 40.09 | ||
| 10-1093-90 | 100µmoles | .08grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
01/11/2012 | http://www.glenres.com/ProductFiles/10-1093.html