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Catalog Number: 10-1085-xx
Description: 2-Amino-dA-CE Phosphoramidite
| 5'-Dimethoxytrityl-N2,N6-bis(diisobutylaminomethylidene)-2,6-diamino- 2'-deoxypurine riboside-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C58H83N10O6P | M.W.: 1047.33 | F.W.: 328.22 |
Diluent: Anhydrous Acetonitrile |
| Coupling: 15 minute coupling recommended.SEE TECHNICAL BULLETIN |
| Deprotection: SEE TECHNICAL BULLETIN (http://www.glenresearch.com/Technical/TB_2-Amino-dA.pdf) Technical Bulletin |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
| Stability in Solution: 2-3 days |
| The convertable nucleoside 10-1071 S6-DNP-dG-CE Phosphoramidite can also be used to incorporate 2-Amino-dA by post synthesis modification of the base after oligonucleotide synthesis. 2-Amino-dA-CED Phosphoramidite has proven to be a more reliable reagent for incorporation of 2-Amino-dA into an oligonucleotide. Deprotection: Acetyl-protected dC must be used during synthesis if the AMA deprotection is to be used or transamination will occur. If benzoyl-dC is used during synthesis ammonium Hydroxide for 7 days at 55oC may be used for deprotection. |
| Catalog Information | Material Safety Data Sheet |
EXTINCTION DATA
| Item | Nucleoside | λMax-1 | Emax-1 | λMax-2 | Emax-2 | E260 |
| (nm) | (ml/µmole) | nm | (ml/µmole) | (ml/µmole) | ||
| 10-1085 | 2,6-diaminoPurine | 278 | 10.2 | 255 | 9.3 | 8.5 |
Literature Highlights
| Glen Report 11.1: USING MODIFIED BASES TO OPTIMIZE HYBRIDIZATION |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | 0.1M Dil. (mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1085-02 | 0.25grams | .25grams | 2.39 | 66.33 | 39.8 | 24.88 | 18.09 | 13.27 | 3.32 |
| 10-1085-90 | 100µmoles | .105grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1085-95 | 50µmoles | .052grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| Expedite | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1085-02 | 0.25grams | .25grams | 3.56 | .067 | 64.8 | 40.5 | 29.45 | 4.05 | |
| 10-1085-90 | 100µmoles | .105grams | 1.5 | .067 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-1085-95 | 50µmoles | .052grams | .75 | .067 | 8.6 | 5.38 | 3.91 | .54 | |
| Beckman | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1085-02 | 0.25grams | .25grams | 3.56 | .067 | 66.4 | 41.5 | 30.18 | ||
| 10-1085-90 | 100µmoles | .105grams | 1.5 | .067 | 25.2 | 15.75 | 11.45 | ||
| 10-1085-95 | 50µmoles | .052grams | .75 | .067 | 10.2 | 6.38 | 4.64 | ||
06/25/2010 | http://www.glenres.com/ProductFiles/10-1085.html