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Catalog Number: 10-1083-xx
Description: 7-deaza-8-aza-dA-CE Phosphoramidite
| 5'-Dimethoxytrityl-N6-dimethylaminomethylidene-8-aza-7-deaza-2'-deoxyAdenosine, 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C43H53N8O6P | M.W.: 808.91 | F.W.: 313.20 |
Diluent: Anhydrous Acetonitrile |
| Coupling: No changes needed from standard method recommended by synthesizer manufacturer. |
| Deprotection: No changes needed from standard method recommended by synthesizer manufacturer. |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 2-3 days |
| Catalog Information | Material Safety Data Sheet |
Literature Highlights
| Glen Report 15.1: MORE AND still MORE NOVEL MINOR BASES |
Frequently Asked Technical Question
QUESTION: Which should I use - psoralen C2 or psoralen C6?
RESPONSE:Psoralen C2 is designed to crosslink to a T residue adjacent to the 3'-terminus of the opposite strand of double stranded DNA. Psoralen C6 is intended to fulfill the same purpose but, with the longer spacer, crosslinks to the triple strand of triplex DNA.
Max excitation=330nm, observed at max 395nm
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | 0.1M Dil. (mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1083-02 | 0.25grams | .25grams | 3.09 | 89.67 | 53.8 | 33.63 | 24.45 | 17.93 | 4.48 |
| 10-1083-90 | 100µmoles | .081grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1083-95 | 50µmoles | .04grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| Expedite | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1083-02 | 0.25grams | .25grams | 4.61 | .067 | 85.8 | 53.63 | 39 | 5.36 | |
| 10-1083-90 | 100µmoles | .081grams | 1.5 | .067 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-1083-95 | 50µmoles | .04grams | .75 | .067 | 8.6 | 5.38 | 3.91 | .54 | |
| Beckman | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1083-02 | 0.25grams | .25grams | 4.61 | .067 | 87.4 | 54.63 | 39.73 | ||
| 10-1083-90 | 100µmoles | .081grams | 1.5 | .067 | 25.2 | 15.75 | 11.45 | ||
| 10-1083-95 | 50µmoles | .04grams | .75 | .067 | 10.2 | 6.38 | 4.64 | ||
11/14/2008 | http://www.glenres.com/ProductFiles/10-1083.html