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Catalog Number: 10-1072-xx
Description: 6-thio-dG-CE Phosphoramidite
| 5'-Dimethoxytrityl-N2-trifluoroacetyl-2'-deoxy-6-(2-cyanoethyl)thio- Guanosine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C45H50F3N8O7PS | M.W.: 934.97 | F.W.: 345.26 |
Diluent: Anhydrous Acetonitrile |
| Coupling: Standard coupling time. Use 0.02 M Iodine for Oxidation. |
| Deprotection: Deprotect with1.0M DBU in anhydrous acetonitrile at Room Temperature for 5hrs to remove the cyanoethyl protection. Complete the deprotection with 50mM NaSH in concentrated NH4OH at Room Temperature for 24hrs. |
| Storage: Refrigerated storage, maximum of 2-8 °C, dry |
| Stability in Solution: 24 hours |
| Catalog Information | Material Safety Data Sheet |
Literature Highlights
| Glen Report 11.1: MORE NOVEL MONOMERS |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | 0.1M Dil. (mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1072-02 | 0.25grams | .25grams | 2.67 | 75.67 | 45.4 | 28.38 | 20.64 | 15.13 | 3.78 |
| 10-1072-90 | 100µmoles | .093grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1072-95 | 50µmoles | .047grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| Expedite | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1072-02 | 0.25grams | .25grams | 3.99 | .067 | 73.4 | 45.88 | 33.36 | 4.59 | |
| 10-1072-90 | 100µmoles | .093grams | 1.5 | .067 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-1072-95 | 50µmoles | .047grams | .75 | .067 | 8.6 | 5.38 | 3.91 | .54 | |
| Beckman | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1072-02 | 0.25grams | .25grams | 3.99 | .067 | 75 | 46.88 | 34.09 | ||
| 10-1072-90 | 100µmoles | .093grams | 1.5 | .067 | 25.2 | 15.75 | 11.45 | ||
| 10-1072-95 | 50µmoles | .047grams | .75 | .067 | 10.2 | 6.38 | 4.64 | ||
11/14/2008 | http://www.glenres.com/ProductFiles/10-1072.html