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Catalog Number: 10-1052-xx
Description: 4-Thio-dU-CE Phosphoramidite
| 5'-Dimethoxytrityl-2'-deoxy-4-(2-cyanoethylthio)-Uridine,3'-[(2-cyanoethyl)- (N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C42H50N5O7PS | M.W.: 799.93 | F.W.: 306.23 |
Diluent: Anhydrous Acetonitrile |
| Coupling: No changes needed from standard method recommended by synthesizer manufacturer. |
| Deprotection: Deprotect with1.0M DBU in anhydrous acetonitrile at Room Temperature for 2hrs to remove the cyanoethyl protection. Complete the deprotection with 50mM NaSH in concentrated NH4OH at Room Temperature for 24hrs. |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
| Stability in Solution: 2-3 days |
| Catalog Information | Material Safety Data Sheet |
EXTINCTION DATA
| Item | Nucleoside | λMax-1 | Emax-1 | λMax-2 | Emax-2 | E260 |
| (nm) | (ml/µmole) | nm | (ml/µmole) | (ml/µmole) | ||
| 10-1052 | 4-thio-dU | 330 | 30.4 | 3.6 |
Literature Highlights
| Glen Report 10.1: MORE NOVEL MONOMERS |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | 0.1M Dil. (mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1052-02 | 0.25grams | .25grams | 3.13 | 91 | 54.6 | 34.13 | 24.82 | 18.2 | 4.55 |
| 10-1052-90 | 100µmoles | .08grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1052-95 | 50µmoles | .04grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| Expedite | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1052-02 | 0.25grams | .25grams | 4.66 | .067 | 86.8 | 54.25 | 39.45 | 5.43 | |
| 10-1052-90 | 100µmoles | .08grams | 1.5 | .067 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-1052-95 | 50µmoles | .04grams | .75 | .067 | 8.6 | 5.38 | 3.91 | .54 | |
| Beckman | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1052-02 | 0.25grams | .25grams | 4.66 | .067 | 88.4 | 55.25 | 40.18 | ||
| 10-1052-90 | 100µmoles | .08grams | 1.5 | .067 | 25.2 | 15.75 | 11.45 | ||
| 10-1052-95 | 50µmoles | .04grams | .75 | .067 | 10.2 | 6.38 | 4.64 | ||
05/03/2010 | http://www.glenres.com/ProductFiles/10-1052.html