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Catalog Number: 10-1046-xx
Description: 2-Aminopurine-CE Phosphoramidite
| 5'-Dimethoxytrityl-N2-(dimethylaminomethylidene)-2'-deoxypurine riboside,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C43H53N8O6P | M.W.: 809.01 | F.W.: 313.21 |
Diluent: Anhydrous Acetonitrile |
| Coupling: No changes needed from standard method recommended by synthesizer manufacturer. |
| Deprotection: No changes needed from standard method recommended by synthesizer manufacturer. |
| Storage: Refrigerated storage, maximum of 2-8 °C, dry |
| Stability in Solution: 2-3 days |
| Catalog Information | Material Safety Data Sheet |
EXTINCTION DATA
| Item | Nucleoside | λMax-1 | Emax-1 | λMax-2 | Emax-2 | E260 |
| (nm) | (ml/µmole) | nm | (ml/µmole) | (ml/µmole) | ||
| 10-1046 | 2-Aminopurine | 303 | 6.8 | 243 | 5.7 | 1 |
Literature Highlights
| Glen Report 6.2: NEW MINOR BASES |
Frequently Asked Technical Question
QUESTION: What are the extinction coefficients for propynyl-dC and dU? Also Halogenated dC and dU derivatives? Others?
RESPONSE:See: Table of Extinction Coefficients
REFERENCE(S):
B. Froehler, Gilead
M. Powell
R. Somers
QUESTION: What are the relative extinction coefficients of 5'-Fluorescein, Hex and Tet etc.. at 260 nm and their Lambda max?
RESPONSE:
| Dye | E 260 nm | E lambda max | Excitation max | Emission max | QY | Notes |
| (L/mol cm) | (L/mol cm) | (nm) | (nm) | |||
| 6-FAM | 21,000 | 75,000 | 494 | 520 | 0.9 | |
| Fluorescein-dT | 38,800 | 75,000 | 494 | 522 | 0.9 | |
| TET | 16,300 | 86,000 | 521 | 541 | 0.9 | |
| HEX | 31,600 | 96,000 | 535 | 553 | 0.7 | |
| TAMRA | 32,300 | 89,000 | 556 | 580 | 0.7 | |
| Cy3 | 5,000 | 150,000 | 546 | 563 | 0.1 | |
| Cy3.5 | 24,000 | 116,000 | 591 | 604 | 0.35 | |
| Cy5 | 10,000 | 250,000 | 646 | 662 | 0.2 | |
| Cy5.5 | 21,500 | 209,000 | 688 | 707 | ||
| acridine | 39,500 | 9,120 | 421 | 497 | ||
| NBD | 3,700 | 19,500 | 485 | 535 | 0.1 | |
| Etheno-dA | 4,800 | 5,800 | 276 | 405 | 0.035 | |
| Furano-dT | 4,000 | 3,700 | 345 | 470 | 0.07/0.02 | QY 0.07 single-stranded; 0.02 ds, deprotected in ammonia 55°C ON |
| 2-aminopurine | 1,000 | 3,600 | 303 | 371 |
REFERENCE(S):
ABI User Bulletin #78, ABI
Oligonucleotide Properties Calculator; http://www.basic.northwestern.edu/biotools/oligocalc.html
QUESTION: What are the relative extinction coefficients of various dyes?
RESPONSE:Please see http://www.glenres.com/Technical/Extinctions.html#dyes
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | 0.1M Dil. (mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1046-02 | 0.25grams | .25grams | 3.09 | 89.67 | 53.8 | 33.63 | 24.45 | 17.93 | 4.48 |
| 10-1046-90 | 100µmoles | .081grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| Expedite | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1046-02 | 0.25grams | .25grams | 4.61 | .067 | 85.8 | 53.63 | 39 | 5.36 | |
| 10-1046-90 | 100µmoles | .081grams | 1.5 | .067 | 23.6 | 14.75 | 10.73 | 1.48 | |
| Beckman | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1046-02 | 0.25grams | .25grams | 4.61 | .067 | 87.4 | 54.63 | 39.73 | ||
| 10-1046-90 | 100µmoles | .081grams | 1.5 | .067 | 25.2 | 15.75 | 11.45 | ||
01/05/2009 | http://www.glenres.com/ProductFiles/10-1046.html