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Catalog Number: 10-1040-xx
Description: dI-CE Phosphoramidite
| 5'-Dimethoxytrityl-2'-deoxyInosine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]- phosphoramidite | ||
| Formula: C40H47N6O7P | M.W.: 754.79 | F.W.: 314.19 |
Diluent: Anhydrous Acetonitrile |
| Coupling: No changes needed from standard method recommended by synthesizer manufacturer. |
| Deprotection: No changes needed from standard method recommended by synthesizer manufacturer. |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
| Stability in Solution: Similar to dA,C,G,T-CE Phosphoramidites |
| Catalog Information | Material Safety Data Sheet |
EXTINCTION DATA
| Item | Nucleoside | λMax-1 | Emax-1 | λMax-2 | Emax-2 | E260 |
| (nm) | (ml/µmole) | nm | (ml/µmole) | (ml/µmole) | ||
| 10-1040 | dI (Inosine) | 249 | 12.5 | 7.5 |
Frequently Asked Technical Question
QUESTION: What is the base-pairing specificity of dI in an oligonucleotide?
RESPONSE:Deoxy-Inosine is often used as a degenerate base in an oligonucleotide probe or primer. This is possible since the structure allows it to base pair with all four bases in various wobble structures. However the base-pairing is not equivalent with each of the 4 naturally occuring bases. The overall preferential order of base-pairing is.
dI-dC > dI-dA > dI-dG = dI-dT
REFERENCE(S):
Martin,F.H. et.al., (1985), Nucleic Acids Res., 13, 8927-8938.
Case-Green,S. C., Southern, E.M., (1994), Nucleic Acids Res., 22, 131-136.
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | 0.1M Dil. (mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1040-02 | 0.25grams | .25grams | 3.31 | 97 | 58.2 | 36.38 | 26.45 | 19.4 | 4.85 |
| 10-1040-90 | 100µmoles | .075grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| Expedite | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1040-02 | 0.25grams | .25grams | 4.94 | .07 | 92.4 | 57.75 | 42 | 5.78 | |
| 10-1040-90 | 100µmoles | .075grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| Beckman | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1040-02 | 0.25grams | .25grams | 4.94 | .07 | 94 | 58.75 | 42.73 | ||
| 10-1040-90 | 100µmoles | .075grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
11/04/2011 | http://www.glenres.com/ProductFiles/10-1040.html