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Catalog Number: 10-1028-xx
Description: 8-oxo-dG-CE Phosphoramidite
| 5'-Dimethoxytrityl-N2-isobutyryl-8-oxo-deoxyGuanosine-3'-[(2-cyanoethyl)- (N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C44H54N7O9P | M.W.: 855.93 | F.W.: 345.21 |
Diluent: Anhydrous Acetonitrile |
| Coupling: No changes needed from standard method recommended by synthesizer manufacturer. |
| Deprotection: Cleave and deprotect with ammonium hydroxide containing 0.25M 2-mercaptoethanol 17 hours at 55°C to avoid oxidative degradation of the 8-oxo-dG site. |
| Storage: Refrigerated storage, maximum of 2-8 °C, dry |
| Stability in Solution: 24 hours |
| Catalog Information | Material Safety Data Sheet |
EXTINCTION DATA
| Item | Nucleoside | λMax-1 | Emax-1 | λMax-2 | Emax-2 | E260 |
| (nm) | (ml/µmole) | nm | (ml/µmole) | (ml/µmole) | ||
| 10-1028 | 8-oxo-dG | 294 | 5.2 | 250 | 6.7 | 5.9 |
Literature Highlights
| Glen Report 7.1: NEW MINOR BASES |
Frequently Asked Technical Question
QUESTION: How do you deprotect 8-oxo-dG?
RESPONSE:Cleave and deprotect with ammonium hydroxide containing 0.25M 2-mercaptoethanol 17 hours at 55°C to avoid oxidative degradation of the 8-oxo-dG site.
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | 0.1M Dil. (mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1028-02 | 0.25grams | .25grams | 2.92 | 84 | 50.4 | 31.5 | 22.91 | 16.8 | 4.2 |
| 10-1028-90 | 100µmoles | .086grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1028-95 | 50µmoles | .043grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| Expedite | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1028-02 | 0.25grams | .25grams | 4.36 | .067 | 80.8 | 50.5 | 36.73 | 5.05 | |
| 10-1028-90 | 100µmoles | .086grams | 1.5 | .067 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-1028-95 | 50µmoles | .043grams | .75 | .067 | 8.6 | 5.38 | 3.91 | .54 | |
| Beckman | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1028-02 | 0.25grams | .25grams | 4.36 | .067 | 82.4 | 51.5 | 37.45 | ||
| 10-1028-90 | 100µmoles | .086grams | 1.5 | .067 | 25.2 | 15.75 | 11.45 | ||
| 10-1028-95 | 50µmoles | .043grams | .75 | .067 | 10.2 | 6.38 | 4.64 | ||
06/02/2009 | http://www.glenres.com/ProductFiles/10-1028.html