New Products - CPR II CPG

CPR II CPG

3'-Phosphate CPG, Catalog No.: 20-2900, (1) in Figure 2, has proved to be a popular and successful product for the preparation of oligonucleotide-3'-phosphates. However, the sulfonylethyl group in this molecule is susceptible to ß-elimination, precluding the ability to conduct base-mediated reactions on the solid support. For example, many researchers treat synthesis supports with a hindered base (e.g., diethylamine, diisopropylethylamine, or DBU) post-synthesis to eliminate and remove the cyanoethyl phosphate groups. In this way, the acrylonitrile formed in situ is removed from the support and is not available to alkylate dT residues at the N3 position in the oligos. Since the sulfonylethyl group in 3'-Phosphate CPG is also susceptible to ß-elimination leading to oligo cleavage, this technique is not compatible with 3'-phosphate CPG.

A more critical example where 3'-phosphate CPG is not compatible is the preparation of 3'-dithiophosphate oligos. These are formed using a phosphate CPG and a thiophosphoramidite in the first cycle. The dithioate is formed by sulfurization using Sulfurizing Reagent II. The synthesis of the oligo is then completed using regular cyanoethyl phosphoramidites. Sulfur is much more susceptible to alkylation by acrylonitrile and regular deprotection would lead to significant alkylation of the 3'-dithioate. Using CPR II CPG, (2) in Figure 2, which is base labile but does not support ß-elimination, the cyanoethyl groups can be removed from the oligo prior to cleavage and base deprotection. This process is illustrated in Figure 3.

We are happy to offer CPR II CPG, Catalog No.: 20-2903, to complement our offering of 3'-Phosphate CPG.

ORDERING INFORMATION -CPR II CPG