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EXPEDITE INSTRUMENTS

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STERLING CE PHOSPHORAMIDITES

Glen Research CE (ß-cyanoethyl) Phosphoramidites are produced and packaged to ensure the highest performance on DNA synthesizers. Every Glen Research product is accompanied by a Certificate of Analysis and HPLC trace, showing the results of our QC testing. Every Glen Research monomer vial is specially cleaned to eliminate particulate contamination.

Item	Catalog No.	Pack	Price ($)
dA-CE Phosphoramidite	10-1000-C2	0.25g	12.50
	10-1000-C5	0.5g	25.00
	10-1000-1C	1.0g	50.00
	10-1000-2C	2.0g	100.00
dC-CE Phosphoramidite	10-1010-C2	0.25g	12.50
	10-1010-C5	0.5g	25.00
	10-1010-1C	1.0g	50.00
	10-1010-2C	2.0g	100.00
Ac-dC-CE Phosphoramidite	10-1015-C2	0.25g	12.50
	10-1015-C5	0.5g	25.00
	10-1015-1C	1.0g	50.00
	10-1015-2C	2.0g	100.00
dG-CE Phosphoramidite	10-1020-C2	0.25g	12.50
	10-1020-C5	0.5g	25.00
	10-1020-1C	1.0g	50.00
	10-1020-2C	2.0g	100.00
dmf-dG-CE Phosphoramidite	10-1029-C2	0.25g	12.50
	10-1029-C5	0.5g	25.00
	10-1029-1C	1.0g	50.00
	10-1029-2C	2.0g	100.00
dT-CE Phosphoramidite	10-1030-C2	0.25g	12.50
	10-1030-C5	0.5g	25.00
	10-1030-1C	1.0g	50.00
	10-1030-2C	2.0g	100.00

QUALITY ASSURANCE

Every batch of these CE Phosphoramidites is tested as follows

HPLC
a) Identity is confirmed by comparison with a reference sample.
b) Purity is determined by HPLC to be ≥98.0%.
TLC
Purity is verified by TLC.
31P NMR
Purity is determined by 31P NMR to be ≥98%.
Coupling Test
Coupling efficiency is determined to be ≥99%.
Solution Test
A 0.1M solution is determined to be clear and free of particulate contamination.
Loss on Drying
Volatile contaminants are determined to be ≤2%.

STERLING SOLVENTS/REAGENTS

All solvents and reagents are prepared to our exacting specifications to ensure the highest synthesis efficiency and are passed through a 0.2 micron filter during packaging to eliminate particulate contamination. Glen Research uses freshly sublimed 1H-tetrazole for premium performance on Expedite synthesizers, but we also offer the more economical solution using crystalline tetrazole. Crystalline tetrazole solutions have been discontinued.

Item	Catalog No.	Pack	Price ($)
Activator
0.45M Tetrazole (sublimed) in Acetonitrile	30-3102-66¹	60mL	50.00
	30-3102-52²	200mL	100.00
	30-3100-57²	450mL	200.00
0.45M Tetrazole (crystalline) in Acetonitrile	30-3112		Discontinued
(Discontinued - above item is the substitute.)	30-3110		Discontinued
Diluent
Acetonitrile, anhydrous	40-4050-45	60mL	12.00
	40-4050-50	100mL	16.00
Anhydrous Wash
Acetonitrile, anhydrous	40-4050-53¹	300mL	40.00
	40-4050-57²	450mL	50.00
Cap Mix A
THF/Ac₂O (9:1)	40-4012-66¹	60mL	15.00
	40-4012-52²	200mL	30.00
	40-4012-57²	450mL	72.00
Cap Mix B
10% MeIm in THF/Pyridine (8:1)	40-4122-66¹	60mL	20.00
	40-4122-52²	200mL	40.00
	40-4122-57²	450mL	96.00
Oxidizing Solution
0.02M I₂in THF/Pyridine/H₂O	40-4132-66¹	60mL	20.00
	40-4132-52²	200mL	40.00
	40-4132-57²	450mL	96.00
Deblocking Mix
3% TCA/DCM	40-4140-68¹	180mL	18.00
	40-4140-71²	1000mL	80.00

ABBREVIATIONS

Ac₂O = Acetic Anhydride
CE = Cyanoethyl
CPG = Controlled Pore Glass
DCM = Dichloromethane
dmf = dimethylformamidine
I₂= Iodine
lcaa = long chain alkylamino
MeIm = 1-Methylimidazole
µm = micromole(s)
nm = nanomole(s)
TCA = Trichloroacetic Acid
THF = Tetrahydrofuran

EXPEDITE INSTRUMENTS

For use on Expedite 8905 instruments.
For use on Expedite 8909 instruments.

STERLING SUPPORTS

All Glen Research supports use the standard long chain alkylamino (lcaa) linker but differ in the glass pore size, 500Å, 1000Å or 2000Å. The 500Å support is appropriate for shorter sequences, while the 1000Å supports perform better in the synthesis of longer (>30-mer) DNA sequences. The 2000Å support is best for very long (>150-mer) oligonucleotides. We have instituted an additional QC test for supports to show the length of oligo that can be prepared before a drop-off in coupling due to steric effects begins to occur. The drop-off point is recorded in the Certificate of Analysis. All Glen Research supports are fully end-capped to ensure that the CPG surface is totally inert, thereby avoiding the introduction of impurity sequences containing deletions at the 3’-terminus.

Catalog No.	Catalog No.	Catalog No.	Catalog No.	Catalog No.	Catalog No.	Catalog No.	Pack	Price($)
dA	dC	dG	dT	dA,dC,dG,dT (1 column of each base)	Ac-dC	dmf-dG
500Å columns
20-2200-42	20-2210-42	20-2220-42	20-2230-42	20-2240-42	20-2213-42		4X0.2�m	40.00
20-2200-41	20-2210-41	20-2220-41	20-2230-41	20-2240-41	20-2213-41		4X1�m	60.00
20-2200-14	20-2210-14	20-2220-14	20-2230-14		20-2213-14		1X15�m	150.00
1000Å Columns
20-2201-45	20-2211-45	20-2221-45	20-2231-45	20-2241-45	20-2215-45	20-2229-45	4X40nm	40.00
20-2201-42	20-2211-42	20-2221-42	20-2231-42	20-2241-42	20-2215-42	20-2229-42	4X0.2�m	40.00
20-2201-41	20-2211-41	20-2221-41	20-2231-41	20-2241-41	20-2215-41	20-2229-41	4X1�m	60.00
20-2201-14	20-2211-14	20-2221-14	20-2231-14		20-2215-14	20-2229-14	1X15�m	150.00
2000Å Columns
20-2202-42	20-2212-42	20-2222-42	20-2232-42	20-2242-42			4X0.2�m	40.00
500Å Bulk CPG
20-2000-01	20-2010-01	20-2020-01	20-2030-01		20-2013-01		0.1g	9.00
20-2000-02	20-2010-02	20-2020-02	20-2030-02		20-2013-02		0.25g	20.00
20-2000-10	20-2010-10	20-2020-10	20-2030-10		20-2013-10		1.0g	75.00
1000Å Bulk CPG
20-2001-01	20-2011-01	20-2021-01	20-2031-01		20-2015-01	20-2029-01	0.1g	9.00
20-2001-02	20-2011-02	20-2021-02	20-2031-02		20-2015-02	20-2029-02	0.25g	20.00
20-2001-10	20-2011-10	20-2021-10	20-2031-10		20-2015-10	20-2029-10	1.0g	75.00
2000Å Bulk CPG
20-2002-01	20-2012-01	20-2022-01	20-2032-01				0.1g	15.00
20-2002-02	20-2012-02	20-2022-02	20-2032-02				0.25g	30.00
20-2002-10	20-2012-10	20-2022-10	20-2032-10				1.0g	105.00

Item	Catalog No.	Pack	Price ($)
Empty Synthesis Columns (40nm, 0.2�m)	20-0021-02	10	48.00
Empty Synthesis Columns (1�m)	20-0021-01	10	48.00
Replacement Filters (40nm, 0.2 or 1�m)	20-0021-0F	20	20.00
Empty Synthesis Columns (15�m) (TWIST™ Style)	20-0040-00	10	300.00
Replacement Frits (15�m)	20-0040-0F	20	30.00

BULK CPG LOADING

500µ supports	35-50�moles/g
1000µ supports	25-40�moles/g
2000µ supports	10-25�moles/g

EXPEDITE INSTRUMENTS

STERLING CE PHOSPHORAMIDITES

QUALITY ASSURANCE

STERLING SOLVENTS/REAGENTS

ABBREVIATIONS

SEE ALSO

EXPEDITE INSTRUMENTS

STERLING SUPPORTS

BULK CPG LOADING

See also