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BECKMAN OLIGO SERIES

STERLING CE PHOSPHORAMIDITES

Glen Research CE (ß-cyanoethyl) Phosphoramidites are produced and packaged to ensure the highest performance on DNA synthesizers. Every Glen Research product is accompanied by a Certificate of Analysis and HPLC trace, showing the results of our QC testing. Every Glen Research monomer vial is specially cleaned to eliminate particulate contamination.

The Beckman catalog numbers are now obsolete. Please use the replacement catalog number when ordering these products. These products wil now ship as the replacement numbers.

Item Catalog No. Pack Price ($)
dA-CE Phosphoramidite 10-1000-B2
Replacement:10-1000-02M		 0.25g 12.50
  10-1000-B5
Replacement:10-1000-05M		 0.5g 25.00
  10-1000-1B
Replacement:10-1000-10M		 1.0g 50.00
dC-CE Phosphoramidite 10-1010-B2
Replacement:10-1010-02M		 0.25g 12.50
  10-1010-B5
Replacement:10-1010-05M		 0.5g 25.00
  10-1010-1B
Replacement:10-1010-10M		 1.0g 50.00
Ac-dC-CE Phosphoramidite 10-1015-B2
Replacement:10-1015-02M		 0.25g 12.50
  10-1015-B5
Replacement:10-1015-05M		 0.5g 25.00
  10-1015-1B
Replacement:10-1015-10M		 1.0g 50.00
dG-CE Phosphoramidite 10-1020-B2
Replacement:10-1020-02M		 0.25g 12.50
  10-1020-B5
Replacement:10-1020-05M		 0.5g 25.00
  10-1020-1B
Replacement:10-1020-10M		 1.0g 50.00
dmf-dG-CE Phosphoramidite 10-1029-B2
Replacement:10-1029-02M		 0.25g 12.50
  10-1029-B5
Replacement:10-1029-05M		 0.5g 25.00
  10-1029-1B
Replacement:10-1029-10M		 1.0g 50.00
dT-CE Phosphoramidite 10-1030-B2
Replacement:10-1030-02M		 0.25g 12.50
  10-1030-B5
Replacement:10-1030-05M		 0.5g 25.00
10-1030-1B
Replacement:10-1030-10M		 1.0g 50.00

QUALITY ASSURANCE

Every batch of these CE Phosphoramidites is tested as follows

  1. HPLC
    a) Identity is confirmed by comparison with a reference sample.
    b) Purity is determined by HPLC to be ≥98.0%.
  2. TLC
    Purity is verified by TLC.
  3. 31P NMR
    Purity is determined by 31P NMR to be ≥98%.
  4. Coupling Test
    Coupling efficiency is determined to be ≥99%.
  5. Solution Test
    A 0.1M solution is determined to be clear and free of particulate contamination.
  6. Loss on Drying
    Volatile contaminants are determined to be ≤2%.

STERLING SOLVENTS/REAGENTS

All solvents and reagents are prepared to our exacting specifications to ensure the highest synthesis efficiency and are passed through a 0.2 micron filter during packaging to eliminate particulate contamination. Glen Research uses freshly sublimed 1H-tetrazole for premium performance on DNA synthesizers.

Item Catalog No.PackPrice ($)
Activator
0.45M Tetrazole in Acetonitrile 30-3100-52 200mL 100.00
Diluent
Acetonitrile, anhydrous 40-4050-45 60mL 12.00
  40-4050-50 100mL 16.00
Cap Mix A
THF/Lutidine/Ac2O (8:1:1) 40-4010-52 200mL 30.00
Cap Mix B
10% MeIm in THF 40-4120-52 200mL 30.00
16% MeIm in THF 40-4220-52 200mL 40.00
Oxidizing Solution
3% I2 in THF/Pyridine/H2O
This item has been discontinued. We suggest 40-4330-xx as the alternative.		 40-4430-52 200mL  
0.02M Iodine in Tetrahydrofuran/Pyridine/Water 40-4330-52 200mL 30.00
Deblocking Mix
3% TCA/DCM 40-4140-57 450mL 36.00

ABBREVIATIONS


Ac2O = Acetic Anhydride
CE = Cyanoethyl
CPG = Controlled Pore Glass
DCM = Dichloromethane
I2 = Iodine
MeIm = 1-Methylimidazole
THF = Tetrahydrofuran
TCA = Trichloroacetic Acid

STERLING SUPPORTS

Oligo 1000 instruments use unique columns available from Beckman Instruments and we recommend these columns. However, Applied Biosystems columns, see Pages 7-8, can be used on Oligo 1000 instruments with the use of an adapter available from Beckman, Part Number 607824.

http://www.glenres.com/Catalog/beckman.html

 
Please contact Glen Research if you have any questions or comments!
 
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