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5'-3' Synthesis Me-Phosphonamidite Me-Phosphoramidite H-Phosphonate Sulfurization ODM

Duplex Stabilization PCR/Sequencing Structural

UltraMILD Terminus Sequence 3'-Modifiers CPR Spacers Dendrimers Photocleavable OPeC

Dabcyl Biotin Fluorescein Rhodamine Cyanine Acridine DNP Cholesterol Psoralen EDTA

TOM RNA Amidites TOM RNA Supports inor Base TOM RNA RNA Amidites RNA Supports Minor Base RNA 2'-OMe-RNA 2'-OMe-Supports Minor 2'-OMe 2'-F-RNA 2'-Se-Me-RNA

ABI Expedite Biosearch 8000 Cyclone Mermade Polyplex Beckman Pharmacia/Amersham UltraMild Alternatives H-Phosphonates Sulfurizing Complete List

NAIM Triphosphates Purification Miscellaneous Physical Properties Index General ;Information

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STERLING CE PHOSPHORAMIDITES

Glen Research CE (ß-cyanoethyl) Phosphoramidites are produced and packaged to ensure the highest performance on DNA synthesizers. Every Glen Research product is accompanied by a Certificate of Analysis and HPLC trace, showing the results of our QC testing. Every Glen Research monomer vial is specially cleaned to eliminate particulate contamination and tested to ensure a tight fit on synthesizers.

Item	Catalog No.	Pack	Price ($)
dA-CE Phosphoramidite	10-1000-02	0.25g	12.50
	10-1000-05	0.5g	25.00
	10-1000-10	1.0g	50.00
	10-1000-20	2.0g	100.00
	10-1000-40	4.0g	200.00
dC-CE Phosphoramidite	10-1010-02	0.25g	12.50
	10-1010-05	0.5g	25.00
	10-1010-10	1.0g	50.00
	10-1010-20	2.0g	100.00
	10-1010-40	4.0g	200.00
Ac-dC-CE Phosphoramidite	10-1015-02	0.25g	12.50
	10-1015-05	0.5g	25.00
	10-1015-10	1.0g	50.00
	10-1015-20	2.0g	100.00
	10-1015-40	4.0g	200.00
dG-CE Phosphoramidite	10-1020-02	0.25g	12.50
	10-1020-05	0.5g	25.00
	10-1020-10	1.0g	50.00
	10-1020-20	2.0g	100.00
	10-1020-40	4.0g	200.00
dmf-dG-CE Phosphoramidite	10-1029-02	0.25g	12.50
	10-1029-05	0.5g	25.00
	10-1029-10	1.0g	50.00
	10-1029-20	2.0g	100.00
	10-1029-40	4.0g	200.00
dT-CE Phosphoramidite	10-1030-02	0.25g	12.50
	10-1030-05	0.5g	25.00
	10-1030-10	1.0g	50.00
	10-1030-20	2.0g	100.00
	10-1030-40	4.0g	200.00

QUALITY ASSURANCE

Every batch of these CE Phosphoramidites is tested as follows

HPLC
a) Identity is confirmed by comparison with a reference sample.
b) Purity is determined by HPLC to be ≥98.0%.
TLC
Purity is verified by TLC.
31P NMR
Purity is determined by 31P NMR to be ≥98%.
Coupling Test
Coupling efficiency is determined to be ≥99%.
Solution Test
A 0.1M solution is determined to be clear and free of particulate contamination.
Loss on Drying
Volatile contaminants are determined to be ≤2%.

dmf-dG-CE Phosphoramidite

STERLING SOLVENTS/REAGENTS

All solvents and reagents are prepared to our exacting specifications to ensure the highest synthesis efficiency and are passed through a 0.2 micron filter during packaging to eliminate particulate contamination. Glen Research uses freshly sublimed 1H-tetrazole for premium performance on Applied Biosystems synthesizers.

Item	Catalog No.	Pack	Price ($)
Activator
0.45M Tetrazole in Acetonitrile	30-3100-45¹	45mL	40.00
	30-3100-52²	200mL	100.00
	30-3100-57³	450mL	200.00
	30-3100-62⁴	2L	760.00
Diluent
Acetonitrile, Anhydrous	40-4050-45	60mL	12.00
	40-4050-50	100mL	16.00
Cap Mix A
THF/Pyridine/Ac₂O	40-4110-45¹	45mL	16.00
	40-4110-52²	200mL	30.00
	40-4110-57³	450mL	72.00
	40-4110-62⁴	2L	325.00
Cap Mix B
16% MeIm in THF	40-4220-45¹	45mL	20.00
(This Cap B solution is identical to the formulation	40-4220-52²	200mL	40.00
produced by Applied Biosystems.)	40-4220-57³	450mL	96.00
	40-4220-62⁴	2L	425.00
Oxidizing Solution
0.1M I₂in THF/Pyridine/H₂O -- This item has been discontinued. We suggest 40-4330-xx as the alternative.	40-4230-52^1,2	200mL
	40-4230-57³	450mL
	40-4230-62⁴	2L
0.02M I₂in THF/Pyridine/H₂O	40-4330-52^1,2	200mL	30.00
	40-4330-57³	450mL	72.00
	40-4330-62⁴	2L	325.00
Deblocking Mix
3% TCA/DCM	40-4140-57^1,2	450mL	36.00
	40-4140-62^3,4	2L	144.00

ABI INSTRUMENTS

45mL septum-capped vials used on oldest ABI 380, 381 and 391 instruments. 200mL oxidizer and 450mL deblock screw-capped bottles also used on ABI 380, 381 and 391 instruments.
Small screw-capped vials used on ABI 392 and 394 instruments.
Larger screw-capped vials used on ABI 392. 394 and 3400 instruments.
Large bottles used on ABI 3900 instruments.

ABBREVIATIONS

Ac₂O = Acetic Anhydride
CE = Cyanoethyl
CPG = Controlled Pore Glass
DCM = Dichloromethane
dmf = dimethylformamidine
I₂= Iodine
lcaa = long chain alkylamino
MeIm = 1-Methylimidazole
µm = micromole(s)
nm = nanomole(s)
TCA = Trichloroacetic Acid
THF = Tetrahydrofuran

All Glen Research CPG supports use the standard long chain alkylamino (lcaa) linker but differ in the glass pore size, 500Å, 1000Å or 2000Å. The 500Å support is appropriate for shorter sequences, while the 1000Å supports perform better in the synthesis of longer (>30-mer) DNA sequences. The 2000Å support is best for very long (>150-mer) oligonucleotides. We have instituted an additional QC test for supports to show the length of oligo that can be prepared before a drop-off in coupling due to steric effects begins to occur. The drop-off point is recorded in the Certificate of Analysis. All Glen Research supports are fully end-capped to ensure that the CPG surface is totally inert, thereby avoiding the introduction of impurity sequences containing deletions at the 3’-terminus.

Catalog No.	Catalog No.	Catalog No.	Catalog No.	Catalog No.	Catalog No.	Catalog No.	Pack	Price($)
dA	dC	dG	dT	dA,dC,dG,dT 1 of each base	Ac-dC	dmf-dG
500Å Columns
20-2100-42	20-2110-42	20-2120-42	20-2130-42	20-2140-42	20-2113-42		4X0.2�m	40.00
20-2100-41	20-2110-41	20-2120-41	20-2130-41	20-2140-41	20-2113-41		4X1�m	60.00
20-2100-13	20-2110-13	20-2120-13	20-2130-13		20-2113-13		1X10�m	100.00
1000Å Columns
20-2101-45	20-2111-45	20-2121-45	20-2131-45	20-2141-45	20-2115-45	20-2129-45	4X40nm	40.00
20-2101-42	20-2111-42	20-2121-42	20-2131-42	20-2141-42	20-2115-42	20-2129-42	4X0.2�m	40.00
20-2101-41	20-2111-41	20-2121-41	20-2131-41	20-2141-41	20-2115-41	20-2129-41	4X1�m	60.00
20-2101-13	20-2111-13	20-2121-13	20-2131-13		20-2115-13	20-2129-13	1X10�m	100.00
2000Å Columns
20-2102-42	20-2112-42	20-2122-42	20-2132-42	20-2142-42			4X0.2�m	40.00
Low Volume (LV) Polystyrene Columns
26-2100-45	26-2110-45	26-2120-45	26-2130-45	26-2140-45			4X40nmm	40.00
26-2100-42	26-2110-42	26-2120-42	26-2130-42	26-2140-42			4X0.2�m	40.00
ABI 3900 Polystyrene Columns
26-2600-65	26-2610-65		26-2630-65			26-2629-65	200X40nm	825.00
26-2600-62	26-2610-62		26-2630-62			26-2629-62	200X200nm	825.00
26-2600-61	26-2610-61		26-2630-61			26-2629-61	200X1�m	927.00
500Å Bulk CPG
20-2000-01	20-2010-01	20-2020-01	20-2030-01		20-2013-01		0.1g	9.00
20-2000-02	20-2010-02	20-2020-02	20-2030-02		20-2013-02		0.25g	20.00
20-2000-10	20-2010-10	20-2020-10	20-2030-10		20-2013-10		1.0g	75.00
1000Å Bulk CPG
20-2001-01	20-2011-01	20-2021-01	20-2031-01		20-2015-01	20-2029-01	0.1g	9.00
20-2001-02	20-2011-02	20-2021-02	20-2031-02		20-2015-02	20-2029-02	0.25g	20.00
20-2001-10	20-2011-10	20-2021-10	20-2031-10		20-2015-10	20-2029-10	1.0g	75.00
2000Å Bulk CPG
20-2002-01	20-2012-01	20-2022-01	20-2032-01				0.1g	15.00
20-2002-02	20-2012-02	20-2022-02	20-2032-02				0.25g	30.00
20-2002-10	20-2012-10	20-2022-10	20-2032-10				1.0g	105.00

Item	Catalog No.	Pack	Price ($)
Empty Synthesis Columns (40nm, 0.2�m, or 1�m) (TWIST™ Style)	20-0030-00	10	60.00
Empty Synthesis Columns (10�m) (TWIST™ Style)	20-0040-00	10	300.00
Replacement Frits (10�m)	20-0040-0F	20	30.00

AB 3900 Polystyrene Modifier Columns

Some of our more popular minor base and modifier supports are available on polystyrene in columns fully compatible with the Applied Biosystems 3900 synthesizer. These include our popular Universal Support III, which will allow DNA, RNA or LNA oligos to be produced on the 3900 with ANY base at the 3’ terminus. At the same time, we are offering 1 µmole columns of Universal Support III for the 3900 instrument. Structures and more complete descriptions are found in the relevant catalog sections for each item. AB 3900 columns can be prepared with virtually any of the CPG supports in this catalog. However, it is usually necessary to adjust the flow using CPG columns since CPG has different flow characteristics from polystyrene.

Item	Catalog No.	Pack	Price ($)
Universal Support III PS
200 nmole columns	26-5110-52	Pack of 10	100.00
40 nmole columns	26-5110-55	Pack of 10	100.00
Universal Support III PS
1 µmole columns	27-5110-51	Pack of 10	150.00
3’-Phosphate PS
200 nmole columns	26-2900-52	Pack of 10	150.00
40 nmole columns	26-2900-55	Pack of 10	150.00
3’-PT-Amino-Modifier C6 PS
200 nmole columns	26-2956-52	Pack of 10	220.00
40 nmole columns	26-2956-55	Pack of 10	220.00
3’-(6-FAM) PS
200 nmole columns	26-2961-52	Pack of 10	300.00
40 nmole columns	26-2961-55	Pack of 10	300.00
3’-Dabcyl PS
200 nmole columns	26-5912-52	Pack of 10	300.00
40 nmole columns	26-5912-55	Pack of 10	300.00
3’-TAMRA PS
200 nmole columns	26-5910-52	Pack of 10	300.00
40 nmole columns	26-5910-55	Pack of 10	300.00
3’-BiotinTEG PS
200 nmole columns	26-2955-52	Pack of 10	300.00
40 nmole columns	26-2955-55	Pack of 10	300.00

BULK CPG LOADING

500� supports	35-50�moles/g
1000� supports	25-40�moles/g
2000� supports	10-25�moles/g